Dual RNA-Seq of Chlamydia and Host Cells View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019-08-06

AUTHORS

James W. Marsh , Regan J. Hayward , Amol Shetty , Anup Mahurkar , Michael S. Humphrys , Garry S. A. Myers

ABSTRACT

During the infection of a host cell by a bacterial pathogen, a cascading series of gene expression changes occurs as each organism manipulates or responds to the other via defense or survival strategies. Unraveling this complex interplay is key for our understanding of bacterial virulence and host response pathways for the development of novel therapeutics. Dual RNA sequencing (dual RNA-Seq) has recently been developed to simultaneously capture host and bacterial transcriptomes from an infected cell. Leveraging the sensitivity and resolution allowed by RNA-seq, dual RNA-Seq can be applied to any bacteria–eukaryotic host interaction. We pioneered dual RNA-Seq to simultaneously capture Chlamydia and host expression profiles during an in vitro infection as proof of principle. Here we provide a detailed laboratory protocol and bioinformatics analysis guidelines for dual RNA-seq experiments focusing on Chlamydia as the organism of interest. More... »

PAGES

123-135

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-9694-0_9

DOI

http://dx.doi.org/10.1007/978-1-4939-9694-0_9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1120140338

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/31385273


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