Reducing Complexity? Cysteine Reduction and S-Alkylation in Proteomic Workflows: Practical Considerations View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019-04-13

AUTHORS

Caroline A. Evans

ABSTRACT

Reduction and alkylation are common processing steps in sample preparation for qualitative and quantitative proteomic analyses. In principle, these steps mitigate the limitations resulting from the presence of disulfide bridges. There has been recurring debate in the proteomics community around their use, with concern over negative impacts that result from overalkylation (off-target, non-thiol sites) or incomplete reduction and/or S-alkylation of cysteine. This chapter integrates findings from a number of studies on different reduction and alkylation strategies, to guide users in experimental design for their optimal use in proteomic workflows. More... »

PAGES

83-97

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-9232-4_7

DOI

http://dx.doi.org/10.1007/978-1-4939-9232-4_7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1113380644

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30980324


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