A Method for Single-Stranded Ancient DNA Library Preparation. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Marie-Theres Gansauge , Matthias Meyer

ABSTRACT

Genomic library preparation from highly degraded DNA is more efficient when library molecules are prepared separately from the complementary strands of DNA fragments. We describe a protocol in which libraries are constructed from single DNA strands in a three-step procedure: single-stranded ligation of the first adapter with T4 DNA ligase in the presence of a splinter oligonucleotide, copying of the DNA strand with a proofreading polymerase, and blunt-end ligation of the second double-stranded adapter with T4 DNA ligase. More... »

PAGES

75-83

Book

TITLE

Ancient DNA

ISBN

978-1-4939-9175-4
978-1-4939-9176-1

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-9176-1_9

DOI

http://dx.doi.org/10.1007/978-1-4939-9176-1_9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112770939

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30875046


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