Double-Stranded Library Preparation for Ancient and Other Degraded Samples. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Kirstin Henneberger , Axel Barlow , Johanna L A Paijmans

ABSTRACT

High-throughput sequencing (HTS) allows fast and cost-efficient sequencing of ancient DNA (aDNA) without prior information about what sequences should be targeted. One necessary step for HTS is the preparation of a sequencing library. Commercial kits are available for this purpose, but many of these are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency. More... »

PAGES

65-73

Book

TITLE

Ancient DNA

ISBN

978-1-4939-9175-4
978-1-4939-9176-1

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-9176-1_8

DOI

http://dx.doi.org/10.1007/978-1-4939-9176-1_8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112770938

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30875045


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