Extraction of Ancient DNA from Plant Remains. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Nathan Wales , Logan Kistler

ABSTRACT

Ancient plant remains from archaeological sites, paleoenvironmental contexts, and herbaria provide excellent opportunities for interrogating plant genetics over Quaternary timescales using ancient DNA (aDNA)-based analyses. A variety of plant tissues, preserved primarily by desiccation and anaerobic waterlogging, have proven to be viable sources of aDNA. Plant tissues are anatomically and chemically diverse and therefore require optimized DNA extraction approaches. Here, we describe a plant DNA isolation protocol that performs well in most contexts. We include recommendations for optimization to retain the very short DNA fragments that are expected to be preserved in degraded tissues. More... »

PAGES

45-55

Book

TITLE

Ancient DNA

ISBN

978-1-4939-9175-4
978-1-4939-9176-1

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-9176-1_6

DOI

http://dx.doi.org/10.1007/978-1-4939-9176-1_6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112770936

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30875043


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