Hybridization Capture of Ancient DNA Using RNA Baits. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

André E R Soares

ABSTRACT

The majority of DNA recovered from ancient remains is derived from organisms that colonize the remains post-mortem, such as soil microbes, or from contaminants, such as DNA from living humans. Additionally, some ancient DNA research projects aim to target specific genomic regions, such as mitochondrial genomes or variable single nucleotide polymorphisms (SNPs). To overcome the challenge of targeting specific fragments of DNA from within a complex DNA extract, methods have been developed to enrich ancient DNA extracts for target DNA relative to nontarget DNA. This chapter describes a method for target DNA enrichment that uses hybridization to biotinylated RNA baits to capture and amplify specific ancient DNA fragments from within the pool of extracted fragments. More... »

PAGES

121-128

Book

TITLE

Ancient DNA

ISBN

978-1-4939-9175-4
978-1-4939-9176-1

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-9176-1_13

DOI

http://dx.doi.org/10.1007/978-1-4939-9176-1_13

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112770943

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30875050


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