Determination of Polycation-Mediated Perturbation of Mitochondrial Respiration in Intact Cells by High-Resolution Respirometry (Oxygraph-2k, OROBOROS). View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Arnaldur Hall , S Moein Moghimi

ABSTRACT

A better understanding of the molecular basis of polycation-mediated impairment of mitochondrial bioenergetics might improve the design and synthesis of more efficient and safer polymeric transfectants. Here we utilize the phosphorylation control protocol for studying the effect of polycations on mitochondrial respiration in intact mammalian cells using Oxygraph-2k (OROBOROS). The protocol offers an opportunity to comprehensively monitor mitochondrial respiration through consecutive additions of various cell membrane permeable compounds that alter mitochondrial respiration, thus providing useful information on different states of mitochondrial respiration. Furthermore, we demonstrate how to analyze the data obtained with the phosphorylation control protocol and how to calculate the respiratory flux ratios, which can be used as indicators of respiratory functionality and mitochondrial health. More... »

PAGES

313-322

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-9092-4_20

DOI

http://dx.doi.org/10.1007/978-1-4939-9092-4_20

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112540072

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30838625


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