Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile Through Extraction and Derivatization of Polar Metabolites View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019-02-07

AUTHORS

Jodi Rattner , Farshad Farshidfar , Oliver F. Bathe

ABSTRACT

Metabolite profiling in complex biological matrices such as serum requires high-throughput technologies capable of accurate and reproducible quantitative analysis and detection of slight differences in metabolite concentrations. Gas chromatography-mass spectrometry (GC-MS) is widely used for characterizing the metabolome. This chapter summarizes the necessary preparatory steps required to profile the metabolome using GC-MS. While this chapter focuses on evaluating polar metabolites in serum samples, the methods can be adapted to quantify nonpolar metabolites in other biological matrices. More... »

PAGES

235-249

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-9027-6_13

DOI

http://dx.doi.org/10.1007/978-1-4939-9027-6_13

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111933678

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30725459


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