Isolation, Differentiation, and Characterization of Human Bone Marrow Stem Cells In Vitro and In Vivo. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Janos Kanczler , Rahul S Tare , Patrick Stumpf , Timothy J Noble , Cameron Black , Richard O C Oreffo

ABSTRACT

In this chapter, we describe techniques for the isolation and characterisation of skeletal stem cells from human bone marrow. The methods for enrichment of STRO-1+ and STRO-4+ cells using magnetic activated cell sorting are described and we also detail techniques for establishing and characterizing osteogenic, adipogenic, and chondrogenic cultures from these cells. Finally, we present methods for studying the ability of these cells to produce bone in vivo using diffusion chambers which have been implanted subcutaneously into mice. More... »

PAGES

53-70

References to SciGraph publications

  • 2015-09. Bone Tissue Engineering in CURRENT MOLECULAR BIOLOGY REPORTS
  • Book

    TITLE

    Bone Research Protocols

    ISBN

    978-1-4939-8996-6
    978-1-4939-8997-3

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-1-4939-8997-3_4

    DOI

    http://dx.doi.org/10.1007/978-1-4939-8997-3_4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1111980790

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30729460


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