Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 β-Cell Line: A Preclinical Model for Type 2 Diabetes. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Jordan Rowlands , Nikita Walz , Joanne E Rowles , Kevin N Keane , Rodrigo Carlessi , Philip Newsholme

ABSTRACT

In type 2 diabetes, prolonged dysregulation of signalling and β-cell metabolic control leads to β-cell dysfunction, and is increasingly associated with abnormal metabolic states which disrupt normal cellular physiology. Utilization of appropriate β-cell models enables a systematic approach to understand the impact of perturbations to the biological system. The BRIN-BD11 β-cell line is a useful, pre-clinical cell model for β-cell dysfunction associated with type 2 diabetes, among other metabolic disorders. The present chapter describes detection and analysis of β-cell dysfunction with respect to changes in bioenergetics and metabolism, generation of intracellular reactive oxygen species, and acute and chronic insulin secretion in the BRIN-BD11 cell line. More... »

PAGES

329-340

References to SciGraph publications

Book

TITLE

Pre-Clinical Models

ISBN

978-1-4939-8993-5
978-1-4939-8994-2

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8994-2_32

DOI

http://dx.doi.org/10.1007/978-1-4939-8994-2_32

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1110506019

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30535710


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