Plant Gene Knockout and Knockdown by CRISPR-Cpf1 (Cas12a) Systems. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Yingxiao Zhang , Yong Zhang , Yiping Qi

ABSTRACT

CRISPR-Cpf1 (Cas12a) is a class II type V endonuclease, which has been used as a genome editing tool in different biological systems. Here we describe a fast, efficient, and user-friendly system for CRISPR-Cpf1 expression vector assembly. In this system, the Pol II promoter is used to drive the expression of both Cpf1 and its crRNA, with the crRNA flanked by hammerhead (HH) and hepatitis delta virus (HDV) ribozyme RNAs for precise crRNA processing. All the components of this system can be modified depending on plant species and experimental goals. Using this system, nearly 100% editing efficiency and 90% gene expression decrease were achieved in rice and Arabidopsis, respectively. More... »

PAGES

245-256

Book

TITLE

Plant Genome Editing with CRISPR Systems

ISBN

978-1-4939-8990-4
978-1-4939-8991-1

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8991-1_18

DOI

http://dx.doi.org/10.1007/978-1-4939-8991-1_18

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111099939

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30610641


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140 https://www.grid.ac/institutes/grid.54549.39 schema:alternateName University of Electronic Science and Technology of China
141 schema:name Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.
142 rdf:type schema:Organization
 




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