Creating Targeted Gene Knockouts in Brassica oleracea Using CRISPR/Cas9. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Tom Lawrenson , Penny Hundleby , Wendy Harwood

ABSTRACT

While public and political views on genetic modification (inserting "foreign" genes to elicit new traits) have resulted in limited exploitation of the technology in some parts of the world, the new era of genome editing (to edit existing genes to gain new traits/genetic variation) has the potential to change the biotech landscape. Genome editing offers a faster and simpler approach to gene knockout in both single and multiple genetic locations, within a single or small number of generations, in a way that has not been possible through alternative breeding methods. Here we describe an Agrobacterium-mediated delivery approach to deliver Cas9 and dual sgRNAs into 4-day-old cotyledonary petioles of Brassica oleracea. Mutations are detected in approximately 10% of primary transgenic plants and go on in subsequent T1 and T2 generations to segregate away from the T-DNA. This enables the recovery of non-transgenic, genome-edited plants carrying a variety of mutations at the target locus. More... »

PAGES

155-170

Book

TITLE

Plant Genome Editing with CRISPR Systems

ISBN

978-1-4939-8990-4
978-1-4939-8991-1

Author Affiliations

From Grant

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8991-1_12

DOI

http://dx.doi.org/10.1007/978-1-4939-8991-1_12

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111099933

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30610635


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