Establishment of Replication-Competent HCV Strain with Minimum Modifications. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Takanobu Kato , Asako Murayama , Takaji Wakita

ABSTRACT

The HCV cell culture system, consisting of the JFH-1 strain and HuH-7 cells, has been broadly used to assess the complete HCV life cycle in cultured cells. However, being able to use multiple HCV strains in such a system is vital for future studies of this virus. We recently established a novel HCV cell culture system using another HCV genotype 2a strain, J6CF, which replicates in chimpanzees but not in cultured cells. We identified effective cell culture-adaptive mutations and established a replication-competent J6CF strain with minimum modifications in cultured cells. The strategy of how we established the replication-competent HCV strain and how we identified the effective cell culture-adaptive mutations is described here and could prove useful for establishing other replication-competent HCV strains. More... »

PAGES

73-83

References to SciGraph publications

Book

TITLE

Hepatitis C Virus Protocols

ISBN

978-1-4939-8975-1
978-1-4939-8976-8

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8976-8_4

DOI

http://dx.doi.org/10.1007/978-1-4939-8976-8_4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1110951362

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30593618


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