Agrobacterium-Mediated Transformation of Barley Immature Embryos. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Alison Hinchliffe , Wendy A Harwood

ABSTRACT

Barley transformation is an essential tool for a range of functional genomics studies as well as for future crop improvement. The demand for efficient crop transformation systems continues to grow, with new genome editing technologies adding to that demand. Here we describe an efficient and routine transformation protocol for the spring barley Golden Promise, based on Agrobacterium-mediated inoculation of immature embryos. This protocol has been widely used for overexpression and RNAi applications and more recently for CRISPR/Cas9 mediated genome editing. Average transformation efficiencies of 25% can be easily achieved. More... »

PAGES

115-126

Book

TITLE

Barley

ISBN

978-1-4939-8942-3
978-1-4939-8944-7

Author Affiliations

From Grant

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8944-7_8

DOI

http://dx.doi.org/10.1007/978-1-4939-8944-7_8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1110063167

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30460562


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