Immunofluorescent Visualization of BMP Signaling Activation on Paraffin-Embedded Tissue Sections. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018-11-10

AUTHORS

Maaike Alkema , Marie-José Goumans , Boudewijn P T Kruithof

ABSTRACT

Immunohistochemistry allows the detection of the presence, localization, and activation of proteins in biological tissues by using the ability of antibodies to bind to specific antigens. Cellular signaling can be visualized using antibodies raised against phosphorylated proteins. Phosphorylated Smad1, Smad5, and Smad9 are the activated signaling molecules of the BMP pathway that transfer BMP signals from the cell surface to the nucleus. Here we describe the detection of phospho-Smad1/5/9 on paraformaldehyde-fixed and paraffin-embedded tissue sections by immunofluorescence. More... »

PAGES

191-200

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8904-1_14

DOI

http://dx.doi.org/10.1007/978-1-4939-8904-1_14

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1109830092

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30414134


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