High-Throughput Image-Based Screening to Identify Chemical Compounds Capable of Activating FOXO View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018-11-10

AUTHORS

Marta Barradas , Wolfgang Link , Diego Megias , Pablo J. Fernandez-Marcos

ABSTRACT

FOXO proteins are transcription factors with important roles in the regulation of the expression of genes involved in cell growth, proliferation, differentiation, and longevity. FOXO proteins are active in the nucleus but, upon post-translational modification they form a docking site for 14-3-3 proteins and are translocated to the cytoplasm where they are inactive.We make use of this regulatory mechanism of FOXO proteins to develop an image-based high-throughput screening platform to detect compounds that regulate FOXO3 subcellular localization. This system has proven a powerful tool to isolate inhibitors of proteins upstream of FOXO, such as PI3K inhibitors. More... »

PAGES

151-161

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8900-3_13

DOI

http://dx.doi.org/10.1007/978-1-4939-8900-3_13

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1109830110

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30414152


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