Identification and Validation of Novel Autophagy Regulators Using an Endogenous Readout siGENOME Screen. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Maria New , Tim Van Acker , Ming Jiang , Rebecca Saunders , Jaclyn S Long , Jun-Ichi Sakamaki , Kevin M Ryan , Michael Howell , Sharon A Tooze

ABSTRACT

Autophagy is a highly regulated process, and its deregulation can contribute to various diseases, including cancer, immune diseases, and neurodegenerative disorders. Here we describe the design, protocol, and analysis of an imaging-based high-throughput screen with an endogenous autophagy readout. The screen uses a genome-wide siRNA library to identify autophagy regulators in mammalian cells. More... »

PAGES

359-374

References to SciGraph publications

Book

TITLE

Autophagy

ISBN

978-1-4939-8872-3
978-1-4939-8873-0

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8873-0_24

DOI

http://dx.doi.org/10.1007/978-1-4939-8873-0_24

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111100008

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30610710


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