Monitoring of Autophagy and Cell Volume Regulation in Kidney Epithelial Cells in Response to Fluid Shear Stress. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Maria M Lazari , Idil Orhon , Patrice Codogno , Nicolas Dupont

ABSTRACT

Fluidic shear stress applied to epithelial cells inside the kidney tubules affects cell size in an autophagy-related manner. Here, we describe the technical equipment that we routinely use to apply shear stress on cells, as well as immunoblotting, immunofluorescence, and three-dimensional cell volume reconstruction techniques used in analysis of the influence of this stress on cells and cellular components. By pointing out details of experimental techniques and potential pitfalls, this review will serve as a guide for those interested in study of how shear stress influences cells. More... »

PAGES

331-340

References to SciGraph publications

Book

TITLE

Autophagy

ISBN

978-1-4939-8872-3
978-1-4939-8873-0

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8873-0_22

DOI

http://dx.doi.org/10.1007/978-1-4939-8873-0_22

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111100006

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30610708


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