Imaging Noncanonical Autophagy and LC3-Associated Phagocytosis in Cultured Cells. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Elise Jacquin , Katherine Fletcher , Oliver Florey

ABSTRACT

Monitoring of ATG8 proteins by western blotting and immunofluorescence microscopy are the most common methods to monitor the autophagy pathway. However, it has recently been shown that ATG8 proteins can be lipidated to non-autophagosome, single-membrane compartments through a noncanonical autophagy pathway. This is commonly found to occur during macro-endocytic processes such as phagocytosis, where it has been termed LC3-associated phagocytosis, and upon lysosomotropic drug treatment. Therefore, care is required when interpreting data based on ATG8 in order to conclude whether a signal relates to the canonical or noncanonical pathway. Here we provide methods to monitor noncanonical autophagy through fluorescence microscopy. More... »

PAGES

295-303

Book

TITLE

Autophagy

ISBN

978-1-4939-8872-3
978-1-4939-8873-0

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8873-0_19

DOI

http://dx.doi.org/10.1007/978-1-4939-8873-0_19

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111100003

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30610705


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150 https://www.grid.ac/institutes/grid.5613.1 schema:alternateName University of Burgundy
151 schema:name INSERM, U1231, Université de Bourgogne Franche Comté, Dijon, France.
152 Signalling Programme, Babraham Institute, Cambridge, UK.
153 rdf:type schema:Organization
 




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