Correlative Light and Electron Microscopy to Analyze LC3 Proteins in Caenorhabditis elegans Embryo. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Céline Largeau , Renaud Legouis

ABSTRACT

In this chapter, we present a protocol to perform correlative light and electron microscopy (CLEM) on Caenorhabditis elegans embryos. We use a specific fixation method which preserves both the GFP fluorescence and the structural integrity of the samples. Thin sections are first analyzed by light microscopy to detect GFP-tagged proteins, then by transmission electron microscopy (TEM) to characterize the ultrastructural anatomy of cells. The superimposition of light and electron images allows to determine the subcellular localization of the fluorescent protein. We have used this method to characterize the roles of autophagy in the phagocytosis of apoptotic cells in C. elegans embryos. We analyzed in apoptotic cell and phagocytic cell the localization of the two homologs of LC3/GABARAP proteins, namely, LGG-1 and LGG-2. More... »

PAGES

281-293

Book

TITLE

Autophagy

ISBN

978-1-4939-8872-3
978-1-4939-8873-0

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8873-0_18

DOI

http://dx.doi.org/10.1007/978-1-4939-8873-0_18

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111100002

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30610704


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