Ultrarapid Sodium Dodecyl Sulfate Polyacrylamide Mini-Gel Electrophoresis. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Rohit Thomas , Biji T Kurien

ABSTRACT

We describe here an ultrafast method for electrophoresing proteins on SDS-PAGE. Previously we reported a method to complete SDS-PAGE and immunoblotting in an hour, including electrophoresing proteins at 70°C in 10 min. Here we show that we can electrophorese molecular weight standards and bovine serum albumin on a 4-20% gradient gel in well under 10 min using heated (44 °C) Laemmli running buffer and high voltage. More... »

PAGES

491-494

References to SciGraph publications

Book

TITLE

Electrophoretic Separation of Proteins

ISBN

978-1-4939-8792-4
978-1-4939-8793-1

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8793-1_42

DOI

http://dx.doi.org/10.1007/978-1-4939-8793-1_42

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1109931129

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30426443


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