Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids. View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2019

AUTHORS

Gyorgy Csako

ABSTRACT

Isoelectric focusing (IEF) coupled with immunodetection (immunofixation or immunoblotting) has become the leading technique for the detection and study of oligoclonal bands (OCBs) in cerebrospinal fluid (CSF) and also is increasingly used in other body fluids such as the tear and serum. Limited commercial availability of precast agarose IEF gels for research and a need for customization prompted reporting a detailed general protocol for the preparation and casting of agarose IEF gel along with sample, control, and isoelectric point marker preparation and carrying out the focusing itself for CSF OCBs. However, the method is readily adaptable to the use of other body fluid specimens and, possibly, research specimens such as culture fluids as well. More... »

PAGES

387-401

References to SciGraph publications

Book

TITLE

Electrophoretic Separation of Proteins

ISBN

978-1-4939-8792-4
978-1-4939-8793-1

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8793-1_33

DOI

http://dx.doi.org/10.1007/978-1-4939-8793-1_33

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1109931120

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30426434


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