Imaging Glioma Progression by Intravital Microscopy View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018-10-13

AUTHORS

Fabio Stanchi , Ken Matsumoto , Holger Gerhardt

ABSTRACT

We describe here a method for generating mouse orthotopic gliomas in order to follow their progression over time by multi-photon laser scanning microscopy. After craniotomy of the parietal bone, glioma cells are implanted in the brain cortex and a glass window is cemented atop, allowing chronical imaging of the tumor. The expression of different fluorescent proteins in tumor cells and in specific cell types of a number of currently available transgenic mouse strains allows obtaining multicolor 3D images of the tumor over time. This technique is suitable both to evaluate the effect of pharmacological treatments and to unravel basic mechanisms of tumor-host interactions. More... »

PAGES

227-243

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8769-6_16

DOI

http://dx.doi.org/10.1007/978-1-4939-8769-6_16

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1107589305

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30315471


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