Detection of Multiple Enzymes in Fermentation Broth Using Single PAGE Analysis View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018

AUTHORS

K. Divakar , J. Deepa Arul Priya , G. Panneer Selvam , M. Suryia Prabha , Ashwin Kannan , G. Nandhini Devi , Pennathur Gautam

ABSTRACT

Activity staining or zymography is a technique to detect enzymes based on their function/activity toward a specific substrate. Multiple enzyme-producing microbes secrete enzymes along with other proteins at varying time points during fermentation. The technique of zymography can be used to detect functionality of enzymes in complex protein/other enzyme mixtures. The protein bands corresponding to specific enzyme among other enzymes/proteins can be located by polyacrylamide gel electrophoresis (PAGE) followed by zymogram analysis. This can be employed to locate the secretion pattern of protein/enzyme from intracellular region to extracellular medium. Here we describe simple method for detection and cellular localization of esterases and protease secreted by single microbial strain in one PAGE gel. More... »

PAGES

133-138

References to SciGraph publications

Book

TITLE

Protein Gel Detection and Imaging

ISBN

978-1-4939-8744-3
978-1-4939-8745-0

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8745-0_16

DOI

http://dx.doi.org/10.1007/978-1-4939-8745-0_16

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1106096745

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30097938


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