Culture and Molecular Profiling of the Respiratory Tract Microbiota View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018-10-05

AUTHORS

Fiona J. Whelan , Laura Rossi , Jennifer C. Stearns , Michael G. Surette

ABSTRACT

Microbiome research of host-associated communities has been advanced recently through improvements in sequencing technologies and bioinformatic methods. Traditional microbiological culture, when combined with molecular techniques, can provide a sensitive platform to comprehensively study the airway microbiota. Here we describe the culture methods necessary to capture a large proportion of the airway microbiota and molecular methods for profiling bacterial communities through the 16S rRNA gene, which, when combined, offer a more complete picture of the diversity of airway microbial communities than either method alone. More... »

PAGES

49-61

Book

TITLE

Microbiome Analysis

ISBN

978-1-4939-8726-9
978-1-4939-8728-3

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8728-3_4

DOI

http://dx.doi.org/10.1007/978-1-4939-8728-3_4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1107501974

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30298247


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