Generation of FLIP and FLIP-FlpE Targeting Vectors for Biallelic Conditional and Reversible Gene Knockouts in Mouse and Human Cells View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018

AUTHORS

Bon-Kyoung Koo

ABSTRACT

Rapid generation of conditional knockout models in a diploid system is challenging. Recently, CRISPR-FLIP strategy has been introduced, which facilitates the generation of biallelic conditional or reversible gene knockouts in various mammalian cell lines including mouse and human pluripotent stem cells by codelivery of the CRISPR/Cas9 system and a universal intronic cassette-FLIP and FLIP-FlpE. Here, I describe the design and cloning method of FLIP and FLIP-FlpE targeting vectors for conditional and reversible gene knockouts. This method is applicable to mouse embryonic stem cells, human induced pluripotent stem cells, and adult stem cell-derived organoids. More... »

PAGES

255-264

References to SciGraph publications

Book

TITLE

Somatic Stem Cells

ISBN

978-1-4939-8696-5
978-1-4939-8697-2

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8697-2_19

DOI

http://dx.doi.org/10.1007/978-1-4939-8697-2_19

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1106894365

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30196416


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