Serpin Phage Display: The Use of a T7 System to Probe Reactive Center Loop Libraries with Different Serine Proteinases View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018-09-08

AUTHORS

Lucas R. de Souza , Benjamin M. Scott , Varsha Bhakta , David A. Donkor , Darian L. Perruzza , William P. Sheffield

ABSTRACT

Phage display is a protein engineering approach that involves construction of libraries of variant proteins displayed on the surface of bacteriophage as capsid fusion proteins and their screening for binding and inhibitory function through the use of bait proteins. Recently, we adapted a commercially available T7 phage display system to create phage-displayed serpin libraries hypervariable in up to five positions in their reactive center loop (RCL). The RCL is a key determinant in serpin specificity, the relationship between the structure of a given serpin and which target proteinase(s) it inhibits. In this chapter, we describe protocols to assess the feasibility of this method for different serpin/proteinase combinations and share experience with this technology gathered in the course of studying two serpins and multiple proteinases with this powerful iterative screening approach. More... »

PAGES

41-64

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8645-3_3

DOI

http://dx.doi.org/10.1007/978-1-4939-8645-3_3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1106835667

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30194592


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacteriophage T7", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptide Library", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Structure, Secondary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Serine Proteases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Serpins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Universidade Federal do ABC", 
          "id": "https://www.grid.ac/institutes/grid.412368.a", 
          "name": [
            "Centre for Natural and Human Sciences, Federal University of ABC (UFABC), S\u00e3o Bernardo do Campo, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "de Souza", 
        "givenName": "Lucas R.", 
        "id": "sg:person.0754256212.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754256212.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Toronto", 
          "id": "https://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Scott", 
        "givenName": "Benjamin M.", 
        "id": "sg:person.01044132032.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044132032.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Canadian Blood Services", 
          "id": "https://www.grid.ac/institutes/grid.423370.1", 
          "name": [
            "Centre for Innovation, Canadian Blood Services, Hamilton, ON, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bhakta", 
        "givenName": "Varsha", 
        "id": "sg:person.01177403453.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177403453.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "McMaster University", 
          "id": "https://www.grid.ac/institutes/grid.25073.33", 
          "name": [
            "Centre for Innovation, Canadian Blood Services, Hamilton, ON, Canada", 
            "Department of Pathology and Molecular Medicine, HSC 4N66, McMaster University, Hamilton, ON, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Donkor", 
        "givenName": "David A.", 
        "id": "sg:person.01123155753.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123155753.94"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Toronto", 
          "id": "https://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Faculty of Medicine, University of Toronto, Toronto, ON, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Perruzza", 
        "givenName": "Darian L.", 
        "id": "sg:person.012701767655.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012701767655.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "McMaster University", 
          "id": "https://www.grid.ac/institutes/grid.25073.33", 
          "name": [
            "Centre for Innovation, Canadian Blood Services, Hamilton, ON, Canada", 
            "Department of Pathology and Molecular Medicine, HSC 4N66, McMaster University, Hamilton, ON, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sheffield", 
        "givenName": "William P.", 
        "id": "sg:person.01063155053.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063155053.70"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0378-1119(93)90164-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001669207"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(93)90164-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001669207"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.9.4808", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001876363"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1139/w98-015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004551028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.2000.4356", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006662845"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biochi.2010.05.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017500933"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35038119", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017892746", 
          "https://doi.org/10.1038/35038119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35038119", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017892746", 
          "https://doi.org/10.1038/35038119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(93)90162-v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020026206"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(93)90162-v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020026206"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.thromres.2005.03.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021262571"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.thromres.2005.03.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021262571"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0129125", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022063957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.97.14.7727", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022920590"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/protein/gzn016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026000990"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-011-3834-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028390165", 
          "https://doi.org/10.1007/s00253-011-3834-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/000114133", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029799849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.275.8.5839", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029877891"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0084491", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030310028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1538-7836.2011.04360.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030322957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/b978-0-12-385950-1.00012-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030443174"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2011.11.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030893060"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2011.11.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030893060"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkt1104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031308453"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejm198309223091203", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034160606"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biochi.2016.05.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037129394"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m305195200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037443530"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/mtna.2014.74", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041820765"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/bc.2005.035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042428798"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci112346", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042527839"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.2003.03963.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044713890"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.2000.4035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046527642"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.94.2.453", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047954896"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s1462399406000184", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048647457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1742-4658.2006.05257.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049399215"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ygeno.2008.07.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049637368"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.r110.112771", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052109047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4939-2020-4_17", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052630524", 
          "https://doi.org/10.1007/978-1-4939-2020-4_17"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.5483/bmbrep.2006.39.1.055", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053662624"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cr010170+", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053971692"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cr010170+", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053971692"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi048833f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055199386"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi048833f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055199386"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0609624", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055202194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0609624", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055202194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8493554", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062655826"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1160/th04-05-0269", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063290180"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1160/th07-03-0197", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063291181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/0929866054395365", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069157629"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077302960", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1995.tb07299.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082438726"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-09-08", 
    "datePublishedReg": "2018-09-08", 
    "description": "Phage display is a protein engineering approach that involves construction of libraries of variant proteins displayed on the surface of bacteriophage as capsid fusion proteins and their screening for binding and inhibitory function through the use of bait proteins. Recently, we adapted a commercially available T7 phage display system to create phage-displayed serpin libraries hypervariable in up to five positions in their reactive center loop (RCL). The RCL is a key determinant in serpin specificity, the relationship between the structure of a given serpin and which target proteinase(s) it inhibits. In this chapter, we describe protocols to assess the feasibility of this method for different serpin/proteinase combinations and share experience with this technology gathered in the course of studying two serpins and multiple proteinases with this powerful iterative screening approach.", 
    "editor": [
      {
        "familyName": "Lucas", 
        "givenName": "Alexandra", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-4939-8645-3_3", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-4939-8644-6", 
        "978-1-4939-8645-3"
      ], 
      "name": "Serpins", 
      "type": "Book"
    }, 
    "name": "Serpin Phage Display: The Use of a T7 System to Probe Reactive Center Loop Libraries with Different Serine Proteinases", 
    "pagination": "41-64", 
    "productId": [
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30194592"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1106835667"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-4939-8645-3_3"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "5c58b722b6e8a7d8f40020e75b7677b69ae5b48652ffb941eff79507b9544f5e"
        ]
      }
    ], 
    "publisher": {
      "location": "New York, NY", 
      "name": "Springer New York", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-4939-8645-3_3", 
      "https://app.dimensions.ai/details/publication/pub.1106835667"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-16T08:30", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000364_0000000364/records_72834_00000001.jsonl", 
    "type": "Chapter", 
    "url": "https://link.springer.com/10.1007%2F978-1-4939-8645-3_3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-8645-3_3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-8645-3_3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-8645-3_3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-8645-3_3'


 

This table displays all metadata directly associated to this object as RDF triples.

274 TRIPLES      23 PREDICATES      77 URIs      27 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-4939-8645-3_3 schema:about N0ef037ad8a33468f8059cf96ccbe6523
2 N394753101ed5420ebc20679edf168a5c
3 N7881780c8f15431a94431e1493be8f4f
4 N9356490920984d5a93f1403ca1d82cf6
5 Ncab45ec8abc74433af0998978a018f03
6 Ncfa9777dff5a41e294b9c8a99094e6db
7 Ne9397b21edc641a7bba8cbe7ac9f960d
8 anzsrc-for:06
9 anzsrc-for:0601
10 schema:author Nbf4ba23695b24c19bf4b533ffabb21f7
11 schema:citation sg:pub.10.1007/978-1-4939-2020-4_17
12 sg:pub.10.1007/s00253-011-3834-x
13 sg:pub.10.1038/35038119
14 https://app.dimensions.ai/details/publication/pub.1077302960
15 https://doi.org/10.1002/j.1460-2075.1995.tb07299.x
16 https://doi.org/10.1006/jmbi.2000.4035
17 https://doi.org/10.1006/jmbi.2000.4356
18 https://doi.org/10.1016/0378-1119(93)90162-v
19 https://doi.org/10.1016/0378-1119(93)90164-x
20 https://doi.org/10.1016/b978-0-12-385950-1.00012-2
21 https://doi.org/10.1016/j.ab.2011.11.005
22 https://doi.org/10.1016/j.biochi.2010.05.003
23 https://doi.org/10.1016/j.biochi.2016.05.006
24 https://doi.org/10.1016/j.thromres.2005.03.004
25 https://doi.org/10.1016/j.ygeno.2008.07.001
26 https://doi.org/10.1017/s1462399406000184
27 https://doi.org/10.1021/bi048833f
28 https://doi.org/10.1021/bi0609624
29 https://doi.org/10.1021/cr010170+
30 https://doi.org/10.1038/mtna.2014.74
31 https://doi.org/10.1056/nejm198309223091203
32 https://doi.org/10.1073/pnas.94.2.453
33 https://doi.org/10.1073/pnas.96.9.4808
34 https://doi.org/10.1073/pnas.97.14.7727
35 https://doi.org/10.1074/jbc.275.8.5839
36 https://doi.org/10.1074/jbc.m305195200
37 https://doi.org/10.1074/jbc.r110.112771
38 https://doi.org/10.1093/nar/gkt1104
39 https://doi.org/10.1093/protein/gzn016
40 https://doi.org/10.1111/j.1432-1033.2003.03963.x
41 https://doi.org/10.1111/j.1538-7836.2011.04360.x
42 https://doi.org/10.1111/j.1742-4658.2006.05257.x
43 https://doi.org/10.1126/science.8493554
44 https://doi.org/10.1139/w98-015
45 https://doi.org/10.1160/th04-05-0269
46 https://doi.org/10.1160/th07-03-0197
47 https://doi.org/10.1172/jci112346
48 https://doi.org/10.1371/journal.pone.0084491
49 https://doi.org/10.1371/journal.pone.0129125
50 https://doi.org/10.1515/bc.2005.035
51 https://doi.org/10.2144/000114133
52 https://doi.org/10.2174/0929866054395365
53 https://doi.org/10.5483/bmbrep.2006.39.1.055
54 schema:datePublished 2018-09-08
55 schema:datePublishedReg 2018-09-08
56 schema:description Phage display is a protein engineering approach that involves construction of libraries of variant proteins displayed on the surface of bacteriophage as capsid fusion proteins and their screening for binding and inhibitory function through the use of bait proteins. Recently, we adapted a commercially available T7 phage display system to create phage-displayed serpin libraries hypervariable in up to five positions in their reactive center loop (RCL). The RCL is a key determinant in serpin specificity, the relationship between the structure of a given serpin and which target proteinase(s) it inhibits. In this chapter, we describe protocols to assess the feasibility of this method for different serpin/proteinase combinations and share experience with this technology gathered in the course of studying two serpins and multiple proteinases with this powerful iterative screening approach.
57 schema:editor N65a69e0fb138467bb304307e584d4470
58 schema:genre chapter
59 schema:inLanguage en
60 schema:isAccessibleForFree false
61 schema:isPartOf N167ea504f5f745b7a81ab351cffe0806
62 schema:name Serpin Phage Display: The Use of a T7 System to Probe Reactive Center Loop Libraries with Different Serine Proteinases
63 schema:pagination 41-64
64 schema:productId N4316592480684cfda7612c8f9a0e0869
65 Nb77afac20daf4ee19e43dc92bf9aed1a
66 Nf567f924967b406894c3674c19d30076
67 Nfa22569d13914c739a31e9258477a9c4
68 schema:publisher Nf65b11f5b5564f65b4825d5b12f00263
69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1106835667
70 https://doi.org/10.1007/978-1-4939-8645-3_3
71 schema:sdDatePublished 2019-04-16T08:30
72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
73 schema:sdPublisher N6d15a74bcf4e4ff9a21ae428846ac02d
74 schema:url https://link.springer.com/10.1007%2F978-1-4939-8645-3_3
75 sgo:license sg:explorer/license/
76 sgo:sdDataset chapters
77 rdf:type schema:Chapter
78 N0ef037ad8a33468f8059cf96ccbe6523 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Serine Proteases
80 rdf:type schema:DefinedTerm
81 N167ea504f5f745b7a81ab351cffe0806 schema:isbn 978-1-4939-8644-6
82 978-1-4939-8645-3
83 schema:name Serpins
84 rdf:type schema:Book
85 N394753101ed5420ebc20679edf168a5c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Humans
87 rdf:type schema:DefinedTerm
88 N4316592480684cfda7612c8f9a0e0869 schema:name dimensions_id
89 schema:value pub.1106835667
90 rdf:type schema:PropertyValue
91 N47c332a314eb475594557cb2a592192c rdf:first sg:person.01044132032.35
92 rdf:rest N71aacc16b1b543d281e0bb356ce2b6bd
93 N65a69e0fb138467bb304307e584d4470 rdf:first N6608de9d10cd4255acb06ab1c149a415
94 rdf:rest rdf:nil
95 N6608de9d10cd4255acb06ab1c149a415 schema:familyName Lucas
96 schema:givenName Alexandra
97 rdf:type schema:Person
98 N6d15a74bcf4e4ff9a21ae428846ac02d schema:name Springer Nature - SN SciGraph project
99 rdf:type schema:Organization
100 N71aacc16b1b543d281e0bb356ce2b6bd rdf:first sg:person.01177403453.15
101 rdf:rest Nce8cfb5f34cb4879a2fadb7e75c4781a
102 N7881780c8f15431a94431e1493be8f4f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Serpins
104 rdf:type schema:DefinedTerm
105 N9356490920984d5a93f1403ca1d82cf6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Bacteriophage T7
107 rdf:type schema:DefinedTerm
108 Nb77afac20daf4ee19e43dc92bf9aed1a schema:name pubmed_id
109 schema:value 30194592
110 rdf:type schema:PropertyValue
111 Nbf4ba23695b24c19bf4b533ffabb21f7 rdf:first sg:person.0754256212.40
112 rdf:rest N47c332a314eb475594557cb2a592192c
113 Ncab45ec8abc74433af0998978a018f03 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Protein Structure, Secondary
115 rdf:type schema:DefinedTerm
116 Nccffc0311a1f4268a699ac9c18ca2331 rdf:first sg:person.01063155053.70
117 rdf:rest rdf:nil
118 Nce8cfb5f34cb4879a2fadb7e75c4781a rdf:first sg:person.01123155753.94
119 rdf:rest Ne70fb2137500433b8071e6ca7cace121
120 Ncfa9777dff5a41e294b9c8a99094e6db schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Animals
122 rdf:type schema:DefinedTerm
123 Ne70fb2137500433b8071e6ca7cace121 rdf:first sg:person.012701767655.01
124 rdf:rest Nccffc0311a1f4268a699ac9c18ca2331
125 Ne9397b21edc641a7bba8cbe7ac9f960d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Peptide Library
127 rdf:type schema:DefinedTerm
128 Nf567f924967b406894c3674c19d30076 schema:name readcube_id
129 schema:value 5c58b722b6e8a7d8f40020e75b7677b69ae5b48652ffb941eff79507b9544f5e
130 rdf:type schema:PropertyValue
131 Nf65b11f5b5564f65b4825d5b12f00263 schema:location New York, NY
132 schema:name Springer New York
133 rdf:type schema:Organisation
134 Nfa22569d13914c739a31e9258477a9c4 schema:name doi
135 schema:value 10.1007/978-1-4939-8645-3_3
136 rdf:type schema:PropertyValue
137 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
138 schema:name Biological Sciences
139 rdf:type schema:DefinedTerm
140 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
141 schema:name Biochemistry and Cell Biology
142 rdf:type schema:DefinedTerm
143 sg:person.01044132032.35 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
144 schema:familyName Scott
145 schema:givenName Benjamin M.
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044132032.35
147 rdf:type schema:Person
148 sg:person.01063155053.70 schema:affiliation https://www.grid.ac/institutes/grid.25073.33
149 schema:familyName Sheffield
150 schema:givenName William P.
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063155053.70
152 rdf:type schema:Person
153 sg:person.01123155753.94 schema:affiliation https://www.grid.ac/institutes/grid.25073.33
154 schema:familyName Donkor
155 schema:givenName David A.
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123155753.94
157 rdf:type schema:Person
158 sg:person.01177403453.15 schema:affiliation https://www.grid.ac/institutes/grid.423370.1
159 schema:familyName Bhakta
160 schema:givenName Varsha
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177403453.15
162 rdf:type schema:Person
163 sg:person.012701767655.01 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
164 schema:familyName Perruzza
165 schema:givenName Darian L.
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012701767655.01
167 rdf:type schema:Person
168 sg:person.0754256212.40 schema:affiliation https://www.grid.ac/institutes/grid.412368.a
169 schema:familyName de Souza
170 schema:givenName Lucas R.
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754256212.40
172 rdf:type schema:Person
173 sg:pub.10.1007/978-1-4939-2020-4_17 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052630524
174 https://doi.org/10.1007/978-1-4939-2020-4_17
175 rdf:type schema:CreativeWork
176 sg:pub.10.1007/s00253-011-3834-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1028390165
177 https://doi.org/10.1007/s00253-011-3834-x
178 rdf:type schema:CreativeWork
179 sg:pub.10.1038/35038119 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017892746
180 https://doi.org/10.1038/35038119
181 rdf:type schema:CreativeWork
182 https://app.dimensions.ai/details/publication/pub.1077302960 schema:CreativeWork
183 https://doi.org/10.1002/j.1460-2075.1995.tb07299.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082438726
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1006/jmbi.2000.4035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046527642
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1006/jmbi.2000.4356 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006662845
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/0378-1119(93)90162-v schema:sameAs https://app.dimensions.ai/details/publication/pub.1020026206
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/0378-1119(93)90164-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1001669207
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/b978-0-12-385950-1.00012-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030443174
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/j.ab.2011.11.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030893060
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/j.biochi.2010.05.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017500933
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1016/j.biochi.2016.05.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037129394
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1016/j.thromres.2005.03.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021262571
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/j.ygeno.2008.07.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049637368
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1017/s1462399406000184 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048647457
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1021/bi048833f schema:sameAs https://app.dimensions.ai/details/publication/pub.1055199386
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1021/bi0609624 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055202194
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1021/cr010170+ schema:sameAs https://app.dimensions.ai/details/publication/pub.1053971692
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1038/mtna.2014.74 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041820765
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1056/nejm198309223091203 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034160606
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1073/pnas.94.2.453 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047954896
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1073/pnas.96.9.4808 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001876363
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1073/pnas.97.14.7727 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022920590
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1074/jbc.275.8.5839 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029877891
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1074/jbc.m305195200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037443530
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1074/jbc.r110.112771 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052109047
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1093/nar/gkt1104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031308453
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1093/protein/gzn016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026000990
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1111/j.1432-1033.2003.03963.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1044713890
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1111/j.1538-7836.2011.04360.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1030322957
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1111/j.1742-4658.2006.05257.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1049399215
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1126/science.8493554 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062655826
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1139/w98-015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004551028
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1160/th04-05-0269 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063290180
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1160/th07-03-0197 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063291181
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1172/jci112346 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042527839
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1371/journal.pone.0084491 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030310028
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1371/journal.pone.0129125 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022063957
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1515/bc.2005.035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042428798
254 rdf:type schema:CreativeWork
255 https://doi.org/10.2144/000114133 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029799849
256 rdf:type schema:CreativeWork
257 https://doi.org/10.2174/0929866054395365 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069157629
258 rdf:type schema:CreativeWork
259 https://doi.org/10.5483/bmbrep.2006.39.1.055 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053662624
260 rdf:type schema:CreativeWork
261 https://www.grid.ac/institutes/grid.17063.33 schema:alternateName University of Toronto
262 schema:name Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada
263 Faculty of Medicine, University of Toronto, Toronto, ON, Canada
264 rdf:type schema:Organization
265 https://www.grid.ac/institutes/grid.25073.33 schema:alternateName McMaster University
266 schema:name Centre for Innovation, Canadian Blood Services, Hamilton, ON, Canada
267 Department of Pathology and Molecular Medicine, HSC 4N66, McMaster University, Hamilton, ON, Canada
268 rdf:type schema:Organization
269 https://www.grid.ac/institutes/grid.412368.a schema:alternateName Universidade Federal do ABC
270 schema:name Centre for Natural and Human Sciences, Federal University of ABC (UFABC), São Bernardo do Campo, Brazil
271 rdf:type schema:Organization
272 https://www.grid.ac/institutes/grid.423370.1 schema:alternateName Canadian Blood Services
273 schema:name Centre for Innovation, Canadian Blood Services, Hamilton, ON, Canada
274 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...