Preparing Frozen-Hydrated Protein–Nucleic Acid Assemblies for High-Resolution Cryo-EM Imaging View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018-06-29

AUTHORS

Panchali Goswami , Julia Locke , Alessandro Costa

ABSTRACT

High-resolution image acquisition and structure determination by cryo-electron microscopy is becoming increasingly streamlined. Preparing electron-microscopy grids of suitable quality remains, however, a critical bottleneck. Strategies to achieve particle monodispersity, optimal sample concentration and suitable ice thickness can vary from specimen to specimen. In this book chapter we describe our protocols for negative-stain grid and cryo-grid preparation, which we apply to studying protein-nucleic acid complexes. More... »

PAGES

287-296

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-8591-3_17

DOI

http://dx.doi.org/10.1007/978-1-4939-8591-3_17

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1105194532

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29956239


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