Cellobiohydrolase (CBH) Activity Assays View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018-06-01

AUTHORS

Hem Kanta Sharma , Wensheng Qin , Chunbao Xu

ABSTRACT

Cellulosic biomass is the most abundant biopolymer on the earth. It has great potential to quench the thirst of liquid energy by producing biofuels and thus help to mitigate human reliance on fossil fuels. Although several cellulase activity assay methods have been used to disintegrate the glycosidic bonds, the appropriate selection of substrates and synergistic involvement of multiple enzymes in hydrolytic activity is not yet fully understood. The proper quantification of hydrolytic enzymes and hydrolysates is challenging because of the heterogeneity of cellulose, changes in enzyme-substrate ratio and the presence of some inhibitory compounds like cellobiose and cellodextran. In the glycosyl hydrolase (GH) family, cellobiohydrolase (CBH) is expected to disrupt the crystalline cellulose and release the sugar molecules. Several methods have been proposed for CBH assay with slight modification in substrate and quantification of hydrolysates. However, the Avicel method is still considered as the most promising and efficient hydrolytic technique so far. The most commonly used CBH assays including Avicel and other recent methods for proper quantification are outlined in this chapter. Also a qualitative screening of CBH producing bacteria using carboxymethyl cellulose (CMC) agar plates is described. More... »

PAGES

185-199

References to SciGraph publications

  • 2009. Cellulase Assays in BIOFUELS
  • 2016-12. Salt-responsive lytic polysaccharide monooxygenases from the mangrove fungus Pestalotiopsis sp. NCi6 in BIOTECHNOLOGY FOR BIOFUELS
  • 2015-11. Characterization of Novel Cellulase-producing Bacteria Isolated From Rotting Wood Samples in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2016. Cellobiohydrolases: Role, Mechanism, and Recent Developments in MICROBIAL ENZYMES IN BIOCONVERSIONS OF BIOMASS
  • 2012-12. Novel enzymes for the degradation of cellulose in BIOTECHNOLOGY FOR BIOFUELS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-1-4939-7877-9_14

    DOI

    http://dx.doi.org/10.1007/978-1-4939-7877-9_14

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1104328729

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29856055


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