Methods to Study Angiogenesis in a Mouse Model of Prostate Cancer View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018-05-22

AUTHORS

Ana-Rita Pedrosa , Alexandre Trindade , António Duarte

ABSTRACT

Angiogenesis is one important hallmark of cancer progression which explains the relevance of developing methods to efficiently analyze the neo-angiogenic process. In this report we make use of the transgenic adenocarcinoma of the murine prostate (TRAMP) model, considered a good model for studying clinical prostate cancer progression, to describe in detail the methods used to study angiogenesis in this type of solid tumor development. In this report we provide step-by-step procedures on the basis of previous work in our laboratory for: the mouse urogenital sinus (UGS) collection; microdissection of the prostate; preparation of the prostatic samples for immunofluorescence (to analyze vascular density, morphology, maturation, functionality, hypoxia, and others); preparation of prostatic samples to histopathological analysis and/or immunohistochemistry; and endothelial and vascular mural cell sorting and isolation by fluorescent associated cell sorting (FACS) to further analysis (mRNA, protein, or other) or to maintain in culture. More... »

PAGES

29-54

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-7845-8_2

DOI

http://dx.doi.org/10.1007/978-1-4939-7845-8_2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1104134133

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29786785


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