Live Single-Cell Mass Spectrometry (LSC-MS) for Plant Metabolomics View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018-05-15

AUTHORS

Keiko Masuda , Yasmine Abouleila , Ahmed Ali , Toshio Yanagida , Tsutomu Masujima

ABSTRACT

Live single-cell mass spectrometry (LSC-MS) allows for the detection of hundreds to thousands of metabolite peaks acquired from a single plant cell within a few minutes. Plant cells are first observed under a stereomicroscope, a cell of interest is chosen, and then sampled using a metal-coated glass microcapillary for subsequent analysis. A few microliters of ionization solvent is then added to the rear end of the capillary followed by the introduction of the capillary’s content directly into the mass spectrometer. High voltage is applied between the capillary and the mass spectrometer inlet to induce nanospray ionization. Metabolite structural confirmation is performed using tandem mass spectrometry analysis (MS/MS) and fragments are matched with MS/MS databases to predict metabolic pathways. This method enables swift and direct molecular detection and identification of specific metabolites from a single plant cell along with their localization within the cell, which will allow for comprehensive understanding of plant metabolomics on a single cell level. More... »

PAGES

269-282

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-7819-9_19

DOI

http://dx.doi.org/10.1007/978-1-4939-7819-9_19

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1103973020

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29761445


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