Targeted LC-MS Analysis for Plant Secondary Metabolites View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018-05-15

AUTHORS

Takafumi Shimizu , Mutsumi Watanabe , Alisdair R. Fernie , Takayuki Tohge

ABSTRACT

Recent technological developments and methodological advances of both liquid chromatography (LC) and mass spectrometry (MS) have allowed LC-MS-based plant metabolomics to become a common tool for investigating quantity, quality, and chemical diversity of plant metabolites. Targeted LC-MS metabolite analysis focuses on the detection and quantitation of the researcher’s target metabolites. Whilst the word “target analysis” has been used for the analytical measurement to obtain the absolute concentrations evaluated by authentic and/or stable-isotope-labeled standards, over time the phrase came to be also used in a broad sense for the measurement of annotatable metabolites by structural information obtained from the combination of different strategies such as MS/MS analysis, reference extracts, mutant analysis and database search. Here, we describe a general protocol for targeted LC-MS metabolite profiling of plant secondary metabolites. Additionally, we introduce some examples of peak annotation using the combination approach. More... »

PAGES

171-181

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-7819-9_12

DOI

http://dx.doi.org/10.1007/978-1-4939-7819-9_12

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1103973013

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29761438


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