Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018

AUTHORS

Darren K. Patten , Giacomo Corleone , Luca Magnani

ABSTRACT

Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) has become an essential tool for epigenetic scientists. ChIP-seq is used to map protein-DNA interactions and epigenetic marks such as histone modifications at the genome-wide level. Here we describe a complete ChIP-seq laboratory protocol (tailored toward processing tissue samples as well as cell lines) and the bioinformatic pipelines utilized for handling raw sequencing files through to peak calling. More... »

PAGES

271-288

Book

TITLE

Epigenome Editing

ISBN

978-1-4939-7773-4
978-1-4939-7774-1

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-7774-1_15

DOI

http://dx.doi.org/10.1007/978-1-4939-7774-1_15

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1101394216

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29524141


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