Ontology type: schema:Chapter
2018
AUTHORSDarren K. Patten , Giacomo Corleone , Luca Magnani
ABSTRACTChromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) has become an essential tool for epigenetic scientists. ChIP-seq is used to map protein-DNA interactions and epigenetic marks such as histone modifications at the genome-wide level. Here we describe a complete ChIP-seq laboratory protocol (tailored toward processing tissue samples as well as cell lines) and the bioinformatic pipelines utilized for handling raw sequencing files through to peak calling. More... »
PAGES271-288
Epigenome Editing
ISBN
978-1-4939-7773-4
978-1-4939-7774-1
http://scigraph.springernature.com/pub.10.1007/978-1-4939-7774-1_15
DOIhttp://dx.doi.org/10.1007/978-1-4939-7774-1_15
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/29524141
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