Directional Collective Migration in Wound Healing Assays View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018-03-11

AUTHORS

Nicolas Molinie , Alexis Gautreau

ABSTRACT

Cell migration is suppressed by confluence in a process called contact inhibition. Relieving contact inhibition upon scratching is one of the simplest ways to induce cell migration in a variety of cell types. Wound healing is probably most relevant to epithelial monolayers, because epithelial cells generally assume a barrier function, which must be restored as fast as possible by the healing process. This versatile assay, however, can also be applied to fibroblasts and to tumor cell types. Furthermore, assessing the cell response to scratch wounding requires no special equipment or reagents. It is one of the few cell migration assays, which can even be performed without videomicroscopy, since the closure of the wound can be estimated at fixed time points. Several hours after wounding, directional collective migration is easily assessed and quantified. However, cell proliferation, which is also induced by the relief of contact inhibition, is one of the confounding factors of wound healing assays that must be taken into account. A recent alternative to the scratch-induced wound is to use special inserts to seed cells into closely spaced chambers. When the insert is removed, contact inhibition is relieved, similar to the scratch-induced wound. In this chapter, we provide the protocol of the two methods and compare their advantages and disadvantages. We also provide a protocol to estimate cell proliferation upon wound healing based on the incorporation of the nucleotide analog EdU. More... »

PAGES

11-19

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-7701-7_2

DOI

http://dx.doi.org/10.1007/978-1-4939-7701-7_2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1101500279

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29525986


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Migration Assays", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Movement", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Wound Healing", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Ecole Polytechnique, Universit\u00e9 Paris-Saclay, BIOC-CNRS UMR7654, Palaiseau, France", 
          "id": "http://www.grid.ac/institutes/grid.10877.39", 
          "name": [
            "Ecole Polytechnique, Universit\u00e9 Paris-Saclay, BIOC-CNRS UMR7654, Palaiseau, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Molinie", 
        "givenName": "Nicolas", 
        "id": "sg:person.01321415033.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321415033.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Biological and Medical Physics, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russian Federation", 
          "id": "http://www.grid.ac/institutes/grid.18763.3b", 
          "name": [
            "Ecole Polytechnique, Universit\u00e9 Paris-Saclay, BIOC-CNRS UMR7654, Palaiseau, France", 
            "School of Biological and Medical Physics, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russian Federation"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gautreau", 
        "givenName": "Alexis", 
        "id": "sg:person.01354164621.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354164621.09"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2018-03-11", 
    "datePublishedReg": "2018-03-11", 
    "description": "Cell migration is suppressed by confluence in a process called contact inhibition. Relieving contact inhibition upon scratching is one of the simplest ways to induce cell migration in a variety of cell types. Wound healing is probably most relevant to epithelial monolayers, because epithelial cells generally assume a barrier function, which must be restored as fast as possible by the healing process. This versatile assay, however, can also be applied to fibroblasts and to tumor cell types. Furthermore, assessing the cell response to scratch wounding requires no special equipment or reagents. It is one of the few cell migration assays, which can even be performed without videomicroscopy, since the closure of the wound can be estimated at fixed time points. Several hours after wounding, directional collective migration is easily assessed and quantified. However, cell proliferation, which is also induced by the relief of contact inhibition, is one of the confounding factors of wound healing assays that must be taken into account. A recent alternative to the scratch-induced wound is to use special inserts to seed cells into closely spaced chambers. When the insert is removed, contact inhibition is relieved, similar to the scratch-induced wound. In this chapter, we provide the protocol of the two methods and compare their advantages and disadvantages. We also provide a protocol to estimate cell proliferation upon wound healing based on the incorporation of the nucleotide analog EdU.", 
    "editor": [
      {
        "familyName": "Gautreau", 
        "givenName": "Alexis", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-4939-7701-7_2", 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-4939-7700-0", 
        "978-1-4939-7701-7"
      ], 
      "name": "Cell Migration", 
      "type": "Book"
    }, 
    "keywords": [
      "wound-healing assays", 
      "healing assays", 
      "wound healing", 
      "cell proliferation", 
      "cell migration", 
      "contact inhibition", 
      "cell types", 
      "cell migration assays", 
      "cell responses", 
      "migration assays", 
      "barrier function", 
      "time points", 
      "epithelial cells", 
      "healing process", 
      "wounds", 
      "inhibition", 
      "epithelial monolayers", 
      "healing", 
      "assays", 
      "nucleotide analogues", 
      "proliferation", 
      "seed cells", 
      "cells", 
      "migration", 
      "videomicroscopy", 
      "relief", 
      "protocol", 
      "fibroblasts", 
      "hours", 
      "versatile assay", 
      "special equipment", 
      "scratching", 
      "response", 
      "closure", 
      "factors", 
      "types", 
      "wounding", 
      "inserts", 
      "function", 
      "analogues", 
      "recent alternative", 
      "confluence", 
      "alternative", 
      "variety", 
      "chamber", 
      "disadvantages", 
      "incorporation", 
      "directional collective migration", 
      "point", 
      "method", 
      "reagents", 
      "process", 
      "monolayers", 
      "collective migration", 
      "chapter", 
      "advantages", 
      "equipment", 
      "way", 
      "simple way", 
      "special insert", 
      "account"
    ], 
    "name": "Directional Collective Migration in Wound Healing Assays", 
    "pagination": "11-19", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1101500279"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-4939-7701-7_2"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "29525986"
        ]
      }
    ], 
    "publisher": {
      "name": "Springer Nature", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-4939-7701-7_2", 
      "https://app.dimensions.ai/details/publication/pub.1101500279"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2022-09-02T16:14", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220902/entities/gbq_results/chapter/chapter_274.jsonl", 
    "type": "Chapter", 
    "url": "https://doi.org/10.1007/978-1-4939-7701-7_2"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7701-7_2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7701-7_2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7701-7_2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7701-7_2'


 

This table displays all metadata directly associated to this object as RDF triples.

155 TRIPLES      22 PREDICATES      90 URIs      83 LITERALS      13 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-4939-7701-7_2 schema:about N0ee2d22d1b39449f8f5243861a4b4fc0
2 N3c1c3c4708974964b0a4b3752c15d5f9
3 N5799f7a9d73246d2b6290f8a7177a62c
4 N79057817242c437aa56dd6cf7f7b480b
5 Ncbaed62d7ff94fbd87700a604b7292c8
6 anzsrc-for:06
7 anzsrc-for:0601
8 schema:author N0de31e7083024d2eb122abe2e63828e8
9 schema:datePublished 2018-03-11
10 schema:datePublishedReg 2018-03-11
11 schema:description Cell migration is suppressed by confluence in a process called contact inhibition. Relieving contact inhibition upon scratching is one of the simplest ways to induce cell migration in a variety of cell types. Wound healing is probably most relevant to epithelial monolayers, because epithelial cells generally assume a barrier function, which must be restored as fast as possible by the healing process. This versatile assay, however, can also be applied to fibroblasts and to tumor cell types. Furthermore, assessing the cell response to scratch wounding requires no special equipment or reagents. It is one of the few cell migration assays, which can even be performed without videomicroscopy, since the closure of the wound can be estimated at fixed time points. Several hours after wounding, directional collective migration is easily assessed and quantified. However, cell proliferation, which is also induced by the relief of contact inhibition, is one of the confounding factors of wound healing assays that must be taken into account. A recent alternative to the scratch-induced wound is to use special inserts to seed cells into closely spaced chambers. When the insert is removed, contact inhibition is relieved, similar to the scratch-induced wound. In this chapter, we provide the protocol of the two methods and compare their advantages and disadvantages. We also provide a protocol to estimate cell proliferation upon wound healing based on the incorporation of the nucleotide analog EdU.
12 schema:editor N7574022fa54b465596c86baa83d25424
13 schema:genre chapter
14 schema:isAccessibleForFree false
15 schema:isPartOf Nbae097df47494b328918293b2344c85f
16 schema:keywords account
17 advantages
18 alternative
19 analogues
20 assays
21 barrier function
22 cell migration
23 cell migration assays
24 cell proliferation
25 cell responses
26 cell types
27 cells
28 chamber
29 chapter
30 closure
31 collective migration
32 confluence
33 contact inhibition
34 directional collective migration
35 disadvantages
36 epithelial cells
37 epithelial monolayers
38 equipment
39 factors
40 fibroblasts
41 function
42 healing
43 healing assays
44 healing process
45 hours
46 incorporation
47 inhibition
48 inserts
49 method
50 migration
51 migration assays
52 monolayers
53 nucleotide analogues
54 point
55 process
56 proliferation
57 protocol
58 reagents
59 recent alternative
60 relief
61 response
62 scratching
63 seed cells
64 simple way
65 special equipment
66 special insert
67 time points
68 types
69 variety
70 versatile assay
71 videomicroscopy
72 way
73 wound healing
74 wound-healing assays
75 wounding
76 wounds
77 schema:name Directional Collective Migration in Wound Healing Assays
78 schema:pagination 11-19
79 schema:productId N23f051b80cdb43b0bf4ca9f9e1612a42
80 Nb16a1c1b55db446c8604d3453ded5477
81 Nef24bfd7b739426a98c227e48ad91f64
82 schema:publisher N395cf6cfea2642ad8586f2a8b03106a3
83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101500279
84 https://doi.org/10.1007/978-1-4939-7701-7_2
85 schema:sdDatePublished 2022-09-02T16:14
86 schema:sdLicense https://scigraph.springernature.com/explorer/license/
87 schema:sdPublisher N6b92933e52934c5f9db2c895a5d37555
88 schema:url https://doi.org/10.1007/978-1-4939-7701-7_2
89 sgo:license sg:explorer/license/
90 sgo:sdDataset chapters
91 rdf:type schema:Chapter
92 N0de31e7083024d2eb122abe2e63828e8 rdf:first sg:person.01321415033.97
93 rdf:rest Naaf12a0951b34dd18605a9eed561e86d
94 N0ee2d22d1b39449f8f5243861a4b4fc0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Wound Healing
96 rdf:type schema:DefinedTerm
97 N23f051b80cdb43b0bf4ca9f9e1612a42 schema:name doi
98 schema:value 10.1007/978-1-4939-7701-7_2
99 rdf:type schema:PropertyValue
100 N395cf6cfea2642ad8586f2a8b03106a3 schema:name Springer Nature
101 rdf:type schema:Organisation
102 N3c1c3c4708974964b0a4b3752c15d5f9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Humans
104 rdf:type schema:DefinedTerm
105 N5799f7a9d73246d2b6290f8a7177a62c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Cell Line, Tumor
107 rdf:type schema:DefinedTerm
108 N6b92933e52934c5f9db2c895a5d37555 schema:name Springer Nature - SN SciGraph project
109 rdf:type schema:Organization
110 N7574022fa54b465596c86baa83d25424 rdf:first Nde77db329f8c49ad94497a93553ffc55
111 rdf:rest rdf:nil
112 N79057817242c437aa56dd6cf7f7b480b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Cell Migration Assays
114 rdf:type schema:DefinedTerm
115 Naaf12a0951b34dd18605a9eed561e86d rdf:first sg:person.01354164621.09
116 rdf:rest rdf:nil
117 Nb16a1c1b55db446c8604d3453ded5477 schema:name pubmed_id
118 schema:value 29525986
119 rdf:type schema:PropertyValue
120 Nbae097df47494b328918293b2344c85f schema:isbn 978-1-4939-7700-0
121 978-1-4939-7701-7
122 schema:name Cell Migration
123 rdf:type schema:Book
124 Ncbaed62d7ff94fbd87700a604b7292c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Cell Movement
126 rdf:type schema:DefinedTerm
127 Nde77db329f8c49ad94497a93553ffc55 schema:familyName Gautreau
128 schema:givenName Alexis
129 rdf:type schema:Person
130 Nef24bfd7b739426a98c227e48ad91f64 schema:name dimensions_id
131 schema:value pub.1101500279
132 rdf:type schema:PropertyValue
133 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
134 schema:name Biological Sciences
135 rdf:type schema:DefinedTerm
136 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
137 schema:name Biochemistry and Cell Biology
138 rdf:type schema:DefinedTerm
139 sg:person.01321415033.97 schema:affiliation grid-institutes:grid.10877.39
140 schema:familyName Molinie
141 schema:givenName Nicolas
142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321415033.97
143 rdf:type schema:Person
144 sg:person.01354164621.09 schema:affiliation grid-institutes:grid.18763.3b
145 schema:familyName Gautreau
146 schema:givenName Alexis
147 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354164621.09
148 rdf:type schema:Person
149 grid-institutes:grid.10877.39 schema:alternateName Ecole Polytechnique, Université Paris-Saclay, BIOC-CNRS UMR7654, Palaiseau, France
150 schema:name Ecole Polytechnique, Université Paris-Saclay, BIOC-CNRS UMR7654, Palaiseau, France
151 rdf:type schema:Organization
152 grid-institutes:grid.18763.3b schema:alternateName School of Biological and Medical Physics, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russian Federation
153 schema:name Ecole Polytechnique, Université Paris-Saclay, BIOC-CNRS UMR7654, Palaiseau, France
154 School of Biological and Medical Physics, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russian Federation
155 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...