GC-MS Analysis of Medium- and Long-Chain Fatty Acids in Blood Samples View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2018

AUTHORS

Lisa R. Hoving , Marieke Heijink , Vanessa van Harmelen , Ko Willems van Dijk , Martin Giera

ABSTRACT

Our body contains a wide variety of fatty acids that differ in chain length, the degree of unsaturation, and location of the double bonds. As the various fatty acids play distinct roles in health and disease, methods that can specifically determine the fatty acid profile are needed for fundamental and clinical studies. Here we describe a method for the separation and quantification of fatty acids ranging from 8 to 24 carbon chain lengths in blood samples using gas chromatography-mass spectrometry following derivatization using pentafluorobenzyl bromide. This method quantitatively monitors fatty acid composition in a manner that satisfies the requirements for comprehensiveness, sensitivity, and accuracy. More... »

PAGES

257-265

References to SciGraph publications

  • 2015-01. Mass-spectrometry-based microbial metabolomics: recent developments and applications in ANALYTICAL AND BIOANALYTICAL CHEMISTRY
  • 2008-02. Membrane lipids: where they are and how they behave in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • Book

    TITLE

    Clinical Metabolomics

    ISBN

    978-1-4939-7591-4
    978-1-4939-7592-1

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-1-4939-7592-1_18

    DOI

    http://dx.doi.org/10.1007/978-1-4939-7592-1_18

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1100590288

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29363079


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