Recovery and Characterization of Bacteria Resisting Infection by Lytic Bacteriophage View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2017-11-09

AUTHORS

Libera Latino , Christine Pourcel

ABSTRACT

Bacteria and bacteriophages coexist and coevolve, bacteriophages being obligatory predators exerting an evolutionary pressure on their prey. Mechanisms in action vary depending on the bacterial genomic content and on the regulation of the bacteriophage cycle. To assess the multiplicity of bacterial genes involved in resistance as well as the changes in the bacteriophage interactions with the bacteria, it is necessary to isolate and investigate large numbers of independent resistant variants. Here we describe protocols that have been applied to the study of Pseudomonas aeruginosa and four of its virulent bacteriophages belonging to the Podoviridae and Myoviridae bacteriophage families. Mutations are identified using whole genome sequencing of resistant variants. Phenotypic analyses are performed to describe the changes conferred by the mutations. More... »

PAGES

85-98

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-7395-8_8

DOI

http://dx.doi.org/10.1007/978-1-4939-7395-8_8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1092593522

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29119434


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