Protein–Protein Interaction Assays with Effector–GFP Fusions in Nicotiana benthamiana View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2017

AUTHORS

Benjamin Petre , Joe Win , Frank L. H. Menke , Sophien Kamoun

ABSTRACT

Plant parasites secrete proteins known as effectors into host tissues to manipulate host cell structures and functions. One of the major goals in effector biology is to determine the host cell compartments and the protein complexes in which effectors accumulate. Here, we describe a five-step pipeline that we routinely use in our lab to achieve this goal, which consists of (1) Golden Gate assembly of pathogen effector-green fluorescent protein (GFP) fusions into binary vectors, (2) Agrobacterium-mediated heterologous protein expression in Nicotiana benthamiana leaf cells, (3) laser-scanning confocal microscopy assay, (4) anti-GFP coimmunoprecipitation-liquid chromatography-tandem mass spectrometry (coIP/MS) assay, and (5) anti-GFP western blotting. This pipeline is suitable for rapid, cost-effective, and medium-throughput screening of pathogen effectors in planta. More... »

PAGES

85-98

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-7249-4_8

DOI

http://dx.doi.org/10.1007/978-1-4939-7249-4_8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1091395562

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28856643


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Agrobacterium tumefaciens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blotting, Western", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatography, Affinity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Green Fluorescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Host-Pathogen Interactions", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunoprecipitation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Confocal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Diseases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Leaves", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Interaction Mapping", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Interaction Maps", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteomics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Fusion Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tandem Mass Spectrometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tobacco", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "The Sainsbury Laboratory"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Petre", 
        "givenName": "Benjamin", 
        "id": "sg:person.0743717567.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0743717567.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "The Sainsbury Laboratory"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Win", 
        "givenName": "Joe", 
        "id": "sg:person.01007636764.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007636764.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "The Sainsbury Laboratory"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Menke", 
        "givenName": "Frank L. H.", 
        "id": "sg:person.01165633757.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01165633757.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "The Sainsbury Laboratory"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kamoun", 
        "givenName": "Sophien", 
        "id": "sg:person.01270641554.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270641554.56"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.1008491107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001234782"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0149035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004778008"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0149035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004778008"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0149035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004778008"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/nph.13532", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005339318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/nph.13532", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005339318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/nph.13532", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005339318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/nph.13532", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005339318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/sqb.2012.77.015933", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011435963"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fpls.2014.00416", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016212124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2013-14-6-211", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017329141", 
          "https://doi.org/10.1186/gb-2013-14-6-211"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12866-016-0632-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046352848", 
          "https://doi.org/10.1186/s12866-016-0632-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-arplant-043014-114623", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047661372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-61737-998-7_15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053568571", 
          "https://doi.org/10.1007/978-1-61737-998-7_15"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-61737-998-7_15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053568571", 
          "https://doi.org/10.1007/978-1-61737-998-7_15"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bst20160044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056718088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bst20160044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056718088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1094/mpmi-01-15-0003-r", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060077375"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1094/mpmi-05-12-0119-fi", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060077612"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1094/mpmi-06-12-0148-ta", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060077694"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1094/mpmi-21-8-1015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060078784"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017", 
    "datePublishedReg": "2017-01-01", 
    "description": "Plant parasites secrete proteins known as effectors into host tissues to manipulate host cell structures and functions. One of the major goals in effector biology is to determine the host cell compartments and the protein complexes in which effectors accumulate. Here, we describe a five-step pipeline that we routinely use in our lab to achieve this goal, which consists of (1) Golden Gate assembly of pathogen effector-green fluorescent protein (GFP) fusions into binary vectors, (2) Agrobacterium-mediated heterologous protein expression in Nicotiana benthamiana leaf cells, (3) laser-scanning confocal microscopy assay, (4) anti-GFP coimmunoprecipitation-liquid chromatography-tandem mass spectrometry (coIP/MS) assay, and (5) anti-GFP western blotting. This pipeline is suitable for rapid, cost-effective, and medium-throughput screening of pathogen effectors in planta.", 
    "editor": [
      {
        "familyName": "Periyannan", 
        "givenName": "Sambasivam", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-4939-7249-4_8", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-4939-7248-7", 
        "978-1-4939-7249-4"
      ], 
      "name": "Wheat Rust Diseases", 
      "type": "Book"
    }, 
    "name": "Protein\u2013Protein Interaction Assays with Effector\u2013GFP Fusions in Nicotiana benthamiana", 
    "pagination": "85-98", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-4939-7249-4_8"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "aa3ef390263ebb89d6964f7a240e373d6832ccb423d692a0e4058fe1424a14bd"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1091395562"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28856643"
        ]
      }
    ], 
    "publisher": {
      "location": "New York, NY", 
      "name": "Springer New York", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-4939-7249-4_8", 
      "https://app.dimensions.ai/details/publication/pub.1091395562"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-15T11:57", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000470.jsonl", 
    "type": "Chapter", 
    "url": "http://link.springer.com/10.1007/978-1-4939-7249-4_8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7249-4_8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7249-4_8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7249-4_8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7249-4_8'


 

This table displays all metadata directly associated to this object as RDF triples.

208 TRIPLES      23 PREDICATES      59 URIs      38 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-4939-7249-4_8 schema:about N045aff2ad99448b9b30a127c21718657
2 N23f9a78bcbb9444eb0a0fea0a7376ff5
3 N2f8e2be9a42e434eabc2dcfc2ee20902
4 N4e8b94a64129455bab450c55507f1ec3
5 N57024a9a03a943c1874cca16ad075783
6 N5e3ed2d402e840c284481419cec958e6
7 N643d28aaec604b0c838d2e395ece96db
8 N7f042e71342e40f386f6d094555a11b8
9 N88cc74da82cc462d9774a4ae7102a724
10 N8d5e4e2bb858415d965b99b104df7af9
11 N9ef08066c2404ad3852f5ef5fe8b28ad
12 Nbee5663c6f4e46d78b8796a2f4e876ae
13 Nc3099bdc8c5940838a7ba91b10a9ad67
14 Nd4997fad81524968a9e4450fcbac44e1
15 Neccceced27d84679b85879cefcd57bfa
16 Nf966e475acd3471fbe3bdd0c78fbb0c4
17 Nf99ece54a8a34ba698d2afcbf0cb3f5f
18 anzsrc-for:06
19 anzsrc-for:0601
20 schema:author Nd3b9ea99fdcf4535990036e91b791466
21 schema:citation sg:pub.10.1007/978-1-61737-998-7_15
22 sg:pub.10.1186/gb-2013-14-6-211
23 sg:pub.10.1186/s12866-016-0632-8
24 https://doi.org/10.1042/bst20160044
25 https://doi.org/10.1073/pnas.1008491107
26 https://doi.org/10.1094/mpmi-01-15-0003-r
27 https://doi.org/10.1094/mpmi-05-12-0119-fi
28 https://doi.org/10.1094/mpmi-06-12-0148-ta
29 https://doi.org/10.1094/mpmi-21-8-1015
30 https://doi.org/10.1101/sqb.2012.77.015933
31 https://doi.org/10.1111/nph.13532
32 https://doi.org/10.1146/annurev-arplant-043014-114623
33 https://doi.org/10.1371/journal.pone.0149035
34 https://doi.org/10.3389/fpls.2014.00416
35 schema:datePublished 2017
36 schema:datePublishedReg 2017-01-01
37 schema:description Plant parasites secrete proteins known as effectors into host tissues to manipulate host cell structures and functions. One of the major goals in effector biology is to determine the host cell compartments and the protein complexes in which effectors accumulate. Here, we describe a five-step pipeline that we routinely use in our lab to achieve this goal, which consists of (1) Golden Gate assembly of pathogen effector-green fluorescent protein (GFP) fusions into binary vectors, (2) Agrobacterium-mediated heterologous protein expression in Nicotiana benthamiana leaf cells, (3) laser-scanning confocal microscopy assay, (4) anti-GFP coimmunoprecipitation-liquid chromatography-tandem mass spectrometry (coIP/MS) assay, and (5) anti-GFP western blotting. This pipeline is suitable for rapid, cost-effective, and medium-throughput screening of pathogen effectors in planta.
38 schema:editor N22d7699cc4094c49a71262306418aa5c
39 schema:genre chapter
40 schema:inLanguage en
41 schema:isAccessibleForFree false
42 schema:isPartOf N185418a7c49a4bbba2865da686f5bde0
43 schema:name Protein–Protein Interaction Assays with Effector–GFP Fusions in Nicotiana benthamiana
44 schema:pagination 85-98
45 schema:productId N2bea4f42254242049c5e80658ec90bb9
46 N36872a21a8194a11b91c9ffa4118fb74
47 Na503e232bab04c638d6395bdc0ab5327
48 Nb4e4b4ce23bf4498afa9c94efec04381
49 schema:publisher N9d5c4ec222cb44259121e120bcf796c6
50 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091395562
51 https://doi.org/10.1007/978-1-4939-7249-4_8
52 schema:sdDatePublished 2019-04-15T11:57
53 schema:sdLicense https://scigraph.springernature.com/explorer/license/
54 schema:sdPublisher N4e1e5a7482044379a77bd0464385924e
55 schema:url http://link.springer.com/10.1007/978-1-4939-7249-4_8
56 sgo:license sg:explorer/license/
57 sgo:sdDataset chapters
58 rdf:type schema:Chapter
59 N045aff2ad99448b9b30a127c21718657 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
60 schema:name Recombinant Fusion Proteins
61 rdf:type schema:DefinedTerm
62 N082b0619efd9429e9f7c0e316c8662a2 rdf:first sg:person.01270641554.56
63 rdf:rest rdf:nil
64 N0d60a892c4d1471ba496525468be1dc3 schema:name The Sainsbury Laboratory
65 rdf:type schema:Organization
66 N185418a7c49a4bbba2865da686f5bde0 schema:isbn 978-1-4939-7248-7
67 978-1-4939-7249-4
68 schema:name Wheat Rust Diseases
69 rdf:type schema:Book
70 N194a616f3d944de3891a4c8cfe1c6a90 rdf:first sg:person.01165633757.36
71 rdf:rest N082b0619efd9429e9f7c0e316c8662a2
72 N22d7699cc4094c49a71262306418aa5c rdf:first Nb912d8a665e04de1806461bcd4a914e4
73 rdf:rest rdf:nil
74 N23f9a78bcbb9444eb0a0fea0a7376ff5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Immunoprecipitation
76 rdf:type schema:DefinedTerm
77 N29f20c5c0fd749f8a18a740462ba0ec4 schema:name The Sainsbury Laboratory
78 rdf:type schema:Organization
79 N2bea4f42254242049c5e80658ec90bb9 schema:name doi
80 schema:value 10.1007/978-1-4939-7249-4_8
81 rdf:type schema:PropertyValue
82 N2f8e2be9a42e434eabc2dcfc2ee20902 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Proteomics
84 rdf:type schema:DefinedTerm
85 N36872a21a8194a11b91c9ffa4118fb74 schema:name readcube_id
86 schema:value aa3ef390263ebb89d6964f7a240e373d6832ccb423d692a0e4058fe1424a14bd
87 rdf:type schema:PropertyValue
88 N4924d639318641d0a378f2f992467238 rdf:first sg:person.01007636764.85
89 rdf:rest N194a616f3d944de3891a4c8cfe1c6a90
90 N4e1e5a7482044379a77bd0464385924e schema:name Springer Nature - SN SciGraph project
91 rdf:type schema:Organization
92 N4e8b94a64129455bab450c55507f1ec3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Plant Leaves
94 rdf:type schema:DefinedTerm
95 N57024a9a03a943c1874cca16ad075783 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Tandem Mass Spectrometry
97 rdf:type schema:DefinedTerm
98 N5e3ed2d402e840c284481419cec958e6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Genetic Vectors
100 rdf:type schema:DefinedTerm
101 N643d28aaec604b0c838d2e395ece96db schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Bacterial Proteins
103 rdf:type schema:DefinedTerm
104 N7f042e71342e40f386f6d094555a11b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Tobacco
106 rdf:type schema:DefinedTerm
107 N88cc74da82cc462d9774a4ae7102a724 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Chromatography, Affinity
109 rdf:type schema:DefinedTerm
110 N8d5e4e2bb858415d965b99b104df7af9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Protein Interaction Mapping
112 rdf:type schema:DefinedTerm
113 N8eb5e01bf0944a75854adaadeb432307 schema:name The Sainsbury Laboratory
114 rdf:type schema:Organization
115 N9d5c4ec222cb44259121e120bcf796c6 schema:location New York, NY
116 schema:name Springer New York
117 rdf:type schema:Organisation
118 N9ef08066c2404ad3852f5ef5fe8b28ad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Microscopy, Confocal
120 rdf:type schema:DefinedTerm
121 Na503e232bab04c638d6395bdc0ab5327 schema:name pubmed_id
122 schema:value 28856643
123 rdf:type schema:PropertyValue
124 Nb4e4b4ce23bf4498afa9c94efec04381 schema:name dimensions_id
125 schema:value pub.1091395562
126 rdf:type schema:PropertyValue
127 Nb912d8a665e04de1806461bcd4a914e4 schema:familyName Periyannan
128 schema:givenName Sambasivam
129 rdf:type schema:Person
130 Nbee5663c6f4e46d78b8796a2f4e876ae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Protein Interaction Maps
132 rdf:type schema:DefinedTerm
133 Nc3099bdc8c5940838a7ba91b10a9ad67 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Agrobacterium tumefaciens
135 rdf:type schema:DefinedTerm
136 Nd3b9ea99fdcf4535990036e91b791466 rdf:first sg:person.0743717567.52
137 rdf:rest N4924d639318641d0a378f2f992467238
138 Nd4997fad81524968a9e4450fcbac44e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Host-Pathogen Interactions
140 rdf:type schema:DefinedTerm
141 Neccceced27d84679b85879cefcd57bfa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Blotting, Western
143 rdf:type schema:DefinedTerm
144 Nf7a389690a0d4805a444fc39ae49a420 schema:name The Sainsbury Laboratory
145 rdf:type schema:Organization
146 Nf966e475acd3471fbe3bdd0c78fbb0c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Green Fluorescent Proteins
148 rdf:type schema:DefinedTerm
149 Nf99ece54a8a34ba698d2afcbf0cb3f5f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Plant Diseases
151 rdf:type schema:DefinedTerm
152 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
153 schema:name Biological Sciences
154 rdf:type schema:DefinedTerm
155 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
156 schema:name Biochemistry and Cell Biology
157 rdf:type schema:DefinedTerm
158 sg:person.01007636764.85 schema:affiliation N0d60a892c4d1471ba496525468be1dc3
159 schema:familyName Win
160 schema:givenName Joe
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007636764.85
162 rdf:type schema:Person
163 sg:person.01165633757.36 schema:affiliation N8eb5e01bf0944a75854adaadeb432307
164 schema:familyName Menke
165 schema:givenName Frank L. H.
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01165633757.36
167 rdf:type schema:Person
168 sg:person.01270641554.56 schema:affiliation N29f20c5c0fd749f8a18a740462ba0ec4
169 schema:familyName Kamoun
170 schema:givenName Sophien
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270641554.56
172 rdf:type schema:Person
173 sg:person.0743717567.52 schema:affiliation Nf7a389690a0d4805a444fc39ae49a420
174 schema:familyName Petre
175 schema:givenName Benjamin
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0743717567.52
177 rdf:type schema:Person
178 sg:pub.10.1007/978-1-61737-998-7_15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053568571
179 https://doi.org/10.1007/978-1-61737-998-7_15
180 rdf:type schema:CreativeWork
181 sg:pub.10.1186/gb-2013-14-6-211 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017329141
182 https://doi.org/10.1186/gb-2013-14-6-211
183 rdf:type schema:CreativeWork
184 sg:pub.10.1186/s12866-016-0632-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046352848
185 https://doi.org/10.1186/s12866-016-0632-8
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1042/bst20160044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056718088
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1073/pnas.1008491107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001234782
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1094/mpmi-01-15-0003-r schema:sameAs https://app.dimensions.ai/details/publication/pub.1060077375
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1094/mpmi-05-12-0119-fi schema:sameAs https://app.dimensions.ai/details/publication/pub.1060077612
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1094/mpmi-06-12-0148-ta schema:sameAs https://app.dimensions.ai/details/publication/pub.1060077694
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1094/mpmi-21-8-1015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060078784
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1101/sqb.2012.77.015933 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011435963
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1111/nph.13532 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005339318
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1146/annurev-arplant-043014-114623 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047661372
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1371/journal.pone.0149035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004778008
206 rdf:type schema:CreativeWork
207 https://doi.org/10.3389/fpls.2014.00416 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016212124
208 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...