Oxime Ether Lipids as Transfection Agents: Assembly and Complexation with siRNA View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2017

AUTHORS

Anu Puri , Serena Zampino , Mathias Viard , Bruce A. Shapiro

ABSTRACT

RNAi-based therapeutic approaches to combat cancer and other diseases are currently an area of great interest. However, practical applications of this approach rely on optimal tools to carry and deliver siRNA to the desired site. Oxime ether lipids (OELs) are a class of molecules among other various carriers being examined for siRNA delivery. OELs, relatively new candidates, belong to a class of non-glycerol based lipids and have begun to claim their place as an siRNA delivery carrier in the field of RNAi therapy. Chemical synthesis steps of OELs are considered relatively simple with the ability to modify the functionalities as desired. OEL-siRNA complexes can be assembled in the presence of serum-containing buffers (or cell culture media) and recent data from our and other groups have demonstrated that OELs are viable carriers for siRNA delivery in the cell culture systems. In this chapter, we provide the details of experimental protocols routinely used in our laboratory to examine OEL-siRNA complexes including their assembly, stability, and transfection efficiencies. More... »

PAGES

241-253

References to SciGraph publications

  • 2000-10. Fluvoxamine in DRUGS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-1-4939-7138-1_16

    DOI

    http://dx.doi.org/10.1007/978-1-4939-7138-1_16

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1090830989

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/28730444


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0306", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Physical Chemistry (incl. Structural)", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Chemical Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line, Tumor", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Ether", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Silencing", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes, Reporter", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Green Fluorescent Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hydrophobic and Hydrophilic Interactions", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Lipids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Liposomes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Oximes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA, Small Interfering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Structure-Activity Relationship", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transfection", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "National Cancer Institute", 
              "id": "https://www.grid.ac/institutes/grid.48336.3a", 
              "name": [
                "National Cancer Institute, National Institutes of Health"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Puri", 
            "givenName": "Anu", 
            "id": "sg:person.0742161657.52", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742161657.52"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National Cancer Institute", 
              "id": "https://www.grid.ac/institutes/grid.48336.3a", 
              "name": [
                "National Cancer Institute, National Institutes of Health"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zampino", 
            "givenName": "Serena", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National Institutes of Health", 
              "id": "https://www.grid.ac/institutes/grid.94365.3d", 
              "name": [
                "National Cancer Institute, National Institutes of Health", 
                "National Institutes of Health"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Viard", 
            "givenName": "Mathias", 
            "id": "sg:person.01047331332.06", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047331332.06"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National Cancer Institute", 
              "id": "https://www.grid.ac/institutes/grid.48336.3a", 
              "name": [
                "National Cancer Institute, National Institutes of Health"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shapiro", 
            "givenName": "Bruce A.", 
            "id": "sg:person.0661403350.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661403350.39"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1021/nn504508s", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006689888"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0005-2736(90)90017-i", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011665997"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0005-2736(90)90017-i", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011665997"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.2165/00003495-200060040-00006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016772894", 
              "https://doi.org/10.2165/00003495-200060040-00006"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.2165/00003495-200060040-00006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016772894", 
              "https://doi.org/10.2165/00003495-200060040-00006"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2217/nnm.15.105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021709676"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1747-0285.2008.00644.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024612566"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/1521-3773(20010601)40:11<2004::aid-anie2004>3.0.co;2-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027810626"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/1521-3773(20010601)40:11<2004::aid-anie2004>3.0.co;2-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027810626"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/cmdc.201100259", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039358327"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/c3ob42270d", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043801043"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bc050138c", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055153882"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bc050138c", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055153882"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/jm060128c", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055950279"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/jm060128c", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055950279"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/jm9704964", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055958424"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/jm9704964", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055958424"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/mp800157x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056213994"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/mp800157x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056213994"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1074625937", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082981289", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2017", 
        "datePublishedReg": "2017-01-01", 
        "description": "RNAi-based therapeutic approaches to combat cancer and other diseases are currently an area of great interest. However, practical applications of this approach rely on optimal tools to carry and deliver siRNA to the desired site. Oxime ether lipids (OELs) are a class of molecules among other various carriers being examined for siRNA delivery. OELs, relatively new candidates, belong to a class of non-glycerol based lipids and have begun to claim their place as an siRNA delivery carrier in the field of RNAi therapy. Chemical synthesis steps of OELs are considered relatively simple with the ability to modify the functionalities as desired. OEL-siRNA complexes can be assembled in the presence of serum-containing buffers (or cell culture media) and recent data from our and other groups have demonstrated that OELs are viable carriers for siRNA delivery in the cell culture systems. In this chapter, we provide the details of experimental protocols routinely used in our laboratory to examine OEL-siRNA complexes including their assembly, stability, and transfection efficiencies.", 
        "editor": [
          {
            "familyName": "Bindewald", 
            "givenName": "Eckart", 
            "type": "Person"
          }, 
          {
            "familyName": "Shapiro", 
            "givenName": "Bruce A.", 
            "type": "Person"
          }
        ], 
        "genre": "chapter", 
        "id": "sg:pub.10.1007/978-1-4939-7138-1_16", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2343593", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": {
          "isbn": [
            "978-1-4939-7137-4", 
            "978-1-4939-7138-1"
          ], 
          "name": "RNA Nanostructures", 
          "type": "Book"
        }, 
        "name": "Oxime Ether Lipids as Transfection Agents: Assembly and Complexation with siRNA", 
        "pagination": "241-253", 
        "productId": [
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/978-1-4939-7138-1_16"
            ]
          }, 
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "62b119c370a7702ebc3d81eb653946099bfeb5e0a6e83da5e95e2bcfaa906824"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1090830989"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "28730444"
            ]
          }
        ], 
        "publisher": {
          "location": "New York, NY", 
          "name": "Springer New York", 
          "type": "Organisation"
        }, 
        "sameAs": [
          "https://doi.org/10.1007/978-1-4939-7138-1_16", 
          "https://app.dimensions.ai/details/publication/pub.1090830989"
        ], 
        "sdDataset": "chapters", 
        "sdDatePublished": "2019-04-15T10:54", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8659_00000436.jsonl", 
        "type": "Chapter", 
        "url": "http://link.springer.com/10.1007/978-1-4939-7138-1_16"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7138-1_16'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7138-1_16'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7138-1_16'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7138-1_16'


     

    This table displays all metadata directly associated to this object as RDF triples.

    197 TRIPLES      23 PREDICATES      56 URIs      35 LITERALS      23 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/978-1-4939-7138-1_16 schema:about N15031cff5c6b4f10bc85577d3835cf0c
    2 N249260d145904ee0bce474acc4b73c3f
    3 N282e958e61e54d94a259a958b4fcf812
    4 N36f68ca7d8d44159b5a7c8edba1c3d13
    5 N6c35b79c295045349ee01a2834ec3661
    6 N6dc097972ad94a2eb500876830853724
    7 N74a892c9473e4df488d2fca5fbc48112
    8 N7e3b6267d0bf4705954eb908da7c578b
    9 N920e156b06da4a85bf6bde7a3e46ab41
    10 Nb25032d1e5374bfcb3592a7575087f83
    11 Nc29edaa10e514c03be9cb4bb1c75d9ea
    12 Nd272b6efba704b02850c7c1e9aa7de60
    13 Ndcd1407721cf4cf7a5d554849ba3223a
    14 Nec0216e7c63b48cda77e7cb17ba58e2e
    15 anzsrc-for:03
    16 anzsrc-for:0306
    17 schema:author Nb5d738b2adec414faeb2ced74603114b
    18 schema:citation sg:pub.10.2165/00003495-200060040-00006
    19 https://app.dimensions.ai/details/publication/pub.1074625937
    20 https://app.dimensions.ai/details/publication/pub.1082981289
    21 https://doi.org/10.1002/1521-3773(20010601)40:11<2004::aid-anie2004>3.0.co;2-5
    22 https://doi.org/10.1002/cmdc.201100259
    23 https://doi.org/10.1016/0005-2736(90)90017-i
    24 https://doi.org/10.1021/bc050138c
    25 https://doi.org/10.1021/jm060128c
    26 https://doi.org/10.1021/jm9704964
    27 https://doi.org/10.1021/mp800157x
    28 https://doi.org/10.1021/nn504508s
    29 https://doi.org/10.1039/c3ob42270d
    30 https://doi.org/10.1111/j.1747-0285.2008.00644.x
    31 https://doi.org/10.2217/nnm.15.105
    32 schema:datePublished 2017
    33 schema:datePublishedReg 2017-01-01
    34 schema:description RNAi-based therapeutic approaches to combat cancer and other diseases are currently an area of great interest. However, practical applications of this approach rely on optimal tools to carry and deliver siRNA to the desired site. Oxime ether lipids (OELs) are a class of molecules among other various carriers being examined for siRNA delivery. OELs, relatively new candidates, belong to a class of non-glycerol based lipids and have begun to claim their place as an siRNA delivery carrier in the field of RNAi therapy. Chemical synthesis steps of OELs are considered relatively simple with the ability to modify the functionalities as desired. OEL-siRNA complexes can be assembled in the presence of serum-containing buffers (or cell culture media) and recent data from our and other groups have demonstrated that OELs are viable carriers for siRNA delivery in the cell culture systems. In this chapter, we provide the details of experimental protocols routinely used in our laboratory to examine OEL-siRNA complexes including their assembly, stability, and transfection efficiencies.
    35 schema:editor Na376827104cb48d5b82ff424ba3285f7
    36 schema:genre chapter
    37 schema:inLanguage en
    38 schema:isAccessibleForFree false
    39 schema:isPartOf Na6c1fe8ec84c45e1801de4d60ae63d10
    40 schema:name Oxime Ether Lipids as Transfection Agents: Assembly and Complexation with siRNA
    41 schema:pagination 241-253
    42 schema:productId N4ff5274ccc38420b83d42ed82c637e45
    43 N5815b08a05f943da90c4842f7ffc4983
    44 Nc1400b5f35214b84b00ffb181bfc1018
    45 Ncb24696fae734290b2829f3e2240c278
    46 schema:publisher N123f757db76b4121ae5931720791b7a0
    47 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090830989
    48 https://doi.org/10.1007/978-1-4939-7138-1_16
    49 schema:sdDatePublished 2019-04-15T10:54
    50 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    51 schema:sdPublisher N21f27dd7d69b4af6852815999b9b8b2c
    52 schema:url http://link.springer.com/10.1007/978-1-4939-7138-1_16
    53 sgo:license sg:explorer/license/
    54 sgo:sdDataset chapters
    55 rdf:type schema:Chapter
    56 N123f757db76b4121ae5931720791b7a0 schema:location New York, NY
    57 schema:name Springer New York
    58 rdf:type schema:Organisation
    59 N15031cff5c6b4f10bc85577d3835cf0c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    60 schema:name Structure-Activity Relationship
    61 rdf:type schema:DefinedTerm
    62 N21f27dd7d69b4af6852815999b9b8b2c schema:name Springer Nature - SN SciGraph project
    63 rdf:type schema:Organization
    64 N249260d145904ee0bce474acc4b73c3f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    65 schema:name Cell Line, Tumor
    66 rdf:type schema:DefinedTerm
    67 N2498830efc304cefaa758e626e134b26 schema:affiliation https://www.grid.ac/institutes/grid.48336.3a
    68 schema:familyName Zampino
    69 schema:givenName Serena
    70 rdf:type schema:Person
    71 N282e958e61e54d94a259a958b4fcf812 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    72 schema:name Transfection
    73 rdf:type schema:DefinedTerm
    74 N33c4fa780ca544a58d4925a78738dc0b rdf:first N2498830efc304cefaa758e626e134b26
    75 rdf:rest N4a87206bd8b145479eac3334216ee66b
    76 N36f68ca7d8d44159b5a7c8edba1c3d13 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    77 schema:name Oximes
    78 rdf:type schema:DefinedTerm
    79 N44b5bc15454948b3893e740a223597ce rdf:first sg:person.0661403350.39
    80 rdf:rest rdf:nil
    81 N4a87206bd8b145479eac3334216ee66b rdf:first sg:person.01047331332.06
    82 rdf:rest N44b5bc15454948b3893e740a223597ce
    83 N4ff5274ccc38420b83d42ed82c637e45 schema:name readcube_id
    84 schema:value 62b119c370a7702ebc3d81eb653946099bfeb5e0a6e83da5e95e2bcfaa906824
    85 rdf:type schema:PropertyValue
    86 N5815b08a05f943da90c4842f7ffc4983 schema:name dimensions_id
    87 schema:value pub.1090830989
    88 rdf:type schema:PropertyValue
    89 N6c35b79c295045349ee01a2834ec3661 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Hydrophobic and Hydrophilic Interactions
    91 rdf:type schema:DefinedTerm
    92 N6dc097972ad94a2eb500876830853724 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Gene Silencing
    94 rdf:type schema:DefinedTerm
    95 N74a892c9473e4df488d2fca5fbc48112 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Liposomes
    97 rdf:type schema:DefinedTerm
    98 N7e3b6267d0bf4705954eb908da7c578b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name Green Fluorescent Proteins
    100 rdf:type schema:DefinedTerm
    101 N920e156b06da4a85bf6bde7a3e46ab41 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Humans
    103 rdf:type schema:DefinedTerm
    104 Na376827104cb48d5b82ff424ba3285f7 rdf:first Nae06c1296d554705b7f22b263a8bec15
    105 rdf:rest Nc83c6fa04f2d4b92aeaf138dbe24cf5b
    106 Na6c1fe8ec84c45e1801de4d60ae63d10 schema:isbn 978-1-4939-7137-4
    107 978-1-4939-7138-1
    108 schema:name RNA Nanostructures
    109 rdf:type schema:Book
    110 Nae06c1296d554705b7f22b263a8bec15 schema:familyName Bindewald
    111 schema:givenName Eckart
    112 rdf:type schema:Person
    113 Nb25032d1e5374bfcb3592a7575087f83 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Gene Expression
    115 rdf:type schema:DefinedTerm
    116 Nb5d738b2adec414faeb2ced74603114b rdf:first sg:person.0742161657.52
    117 rdf:rest N33c4fa780ca544a58d4925a78738dc0b
    118 Nc1400b5f35214b84b00ffb181bfc1018 schema:name pubmed_id
    119 schema:value 28730444
    120 rdf:type schema:PropertyValue
    121 Nc29edaa10e514c03be9cb4bb1c75d9ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name RNA, Small Interfering
    123 rdf:type schema:DefinedTerm
    124 Nc83c6fa04f2d4b92aeaf138dbe24cf5b rdf:first Ncbf67ba685d2416889e24aa4fcd14a7c
    125 rdf:rest rdf:nil
    126 Ncb24696fae734290b2829f3e2240c278 schema:name doi
    127 schema:value 10.1007/978-1-4939-7138-1_16
    128 rdf:type schema:PropertyValue
    129 Ncbf67ba685d2416889e24aa4fcd14a7c schema:familyName Shapiro
    130 schema:givenName Bruce A.
    131 rdf:type schema:Person
    132 Nd272b6efba704b02850c7c1e9aa7de60 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Ether
    134 rdf:type schema:DefinedTerm
    135 Ndcd1407721cf4cf7a5d554849ba3223a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    136 schema:name Genes, Reporter
    137 rdf:type schema:DefinedTerm
    138 Nec0216e7c63b48cda77e7cb17ba58e2e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    139 schema:name Lipids
    140 rdf:type schema:DefinedTerm
    141 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
    142 schema:name Chemical Sciences
    143 rdf:type schema:DefinedTerm
    144 anzsrc-for:0306 schema:inDefinedTermSet anzsrc-for:
    145 schema:name Physical Chemistry (incl. Structural)
    146 rdf:type schema:DefinedTerm
    147 sg:grant.2343593 http://pending.schema.org/fundedItem sg:pub.10.1007/978-1-4939-7138-1_16
    148 rdf:type schema:MonetaryGrant
    149 sg:person.01047331332.06 schema:affiliation https://www.grid.ac/institutes/grid.94365.3d
    150 schema:familyName Viard
    151 schema:givenName Mathias
    152 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047331332.06
    153 rdf:type schema:Person
    154 sg:person.0661403350.39 schema:affiliation https://www.grid.ac/institutes/grid.48336.3a
    155 schema:familyName Shapiro
    156 schema:givenName Bruce A.
    157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661403350.39
    158 rdf:type schema:Person
    159 sg:person.0742161657.52 schema:affiliation https://www.grid.ac/institutes/grid.48336.3a
    160 schema:familyName Puri
    161 schema:givenName Anu
    162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742161657.52
    163 rdf:type schema:Person
    164 sg:pub.10.2165/00003495-200060040-00006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016772894
    165 https://doi.org/10.2165/00003495-200060040-00006
    166 rdf:type schema:CreativeWork
    167 https://app.dimensions.ai/details/publication/pub.1074625937 schema:CreativeWork
    168 https://app.dimensions.ai/details/publication/pub.1082981289 schema:CreativeWork
    169 https://doi.org/10.1002/1521-3773(20010601)40:11<2004::aid-anie2004>3.0.co;2-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027810626
    170 rdf:type schema:CreativeWork
    171 https://doi.org/10.1002/cmdc.201100259 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039358327
    172 rdf:type schema:CreativeWork
    173 https://doi.org/10.1016/0005-2736(90)90017-i schema:sameAs https://app.dimensions.ai/details/publication/pub.1011665997
    174 rdf:type schema:CreativeWork
    175 https://doi.org/10.1021/bc050138c schema:sameAs https://app.dimensions.ai/details/publication/pub.1055153882
    176 rdf:type schema:CreativeWork
    177 https://doi.org/10.1021/jm060128c schema:sameAs https://app.dimensions.ai/details/publication/pub.1055950279
    178 rdf:type schema:CreativeWork
    179 https://doi.org/10.1021/jm9704964 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055958424
    180 rdf:type schema:CreativeWork
    181 https://doi.org/10.1021/mp800157x schema:sameAs https://app.dimensions.ai/details/publication/pub.1056213994
    182 rdf:type schema:CreativeWork
    183 https://doi.org/10.1021/nn504508s schema:sameAs https://app.dimensions.ai/details/publication/pub.1006689888
    184 rdf:type schema:CreativeWork
    185 https://doi.org/10.1039/c3ob42270d schema:sameAs https://app.dimensions.ai/details/publication/pub.1043801043
    186 rdf:type schema:CreativeWork
    187 https://doi.org/10.1111/j.1747-0285.2008.00644.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024612566
    188 rdf:type schema:CreativeWork
    189 https://doi.org/10.2217/nnm.15.105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021709676
    190 rdf:type schema:CreativeWork
    191 https://www.grid.ac/institutes/grid.48336.3a schema:alternateName National Cancer Institute
    192 schema:name National Cancer Institute, National Institutes of Health
    193 rdf:type schema:Organization
    194 https://www.grid.ac/institutes/grid.94365.3d schema:alternateName National Institutes of Health
    195 schema:name National Cancer Institute, National Institutes of Health
    196 National Institutes of Health
    197 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...