Identifying Follicular Regulatory T Cells by Confocal Microscopy View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2017

AUTHORS

Ine Vanderleyden , Michelle A. Linterman

ABSTRACT

Follicular regulatory T cells are a subset of Foxp3+ regulatory T cells that migrate into the B cell follicle after infection or immunization and modulate the germinal center response. The anatomical positioning of follicular regulatory T cells within the germinal center is a defining characteristic of this subset of regulatory T cells; because of this, it is critical that studies of follicular regulatory T cells are able to identify them in situ. In this chapter we describe an immunofluorescence staining method to visualize follicular regulatory T cells in frozen secondary lymphoid tissue sections by confocal imaging. More... »

PAGES

87-93

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-7095-7_7

DOI

http://dx.doi.org/10.1007/978-1-4939-7095-7_7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1085906577

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28589349


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomarkers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescent Antibody Technique", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Germinal Center", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Confocal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spleen", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocyte Subsets", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocytes, Regulatory", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Lymphocyte Signalling and Development, Babraham Institute, Babraham Research Campus"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vanderleyden", 
        "givenName": "Ine", 
        "id": "sg:person.0631204154.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0631204154.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Lymphocyte Signalling and Development, Babraham Institute, Babraham Research Campus"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Linterman", 
        "givenName": "Michelle A.", 
        "id": "sg:person.01137254350.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137254350.67"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nm.2425", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016362145", 
          "https://doi.org/10.1038/nm.2425"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/eji.201546094", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019099760"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13075-014-0471-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033648211", 
          "https://doi.org/10.1186/s13075-014-0471-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13075-014-0471-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033648211", 
          "https://doi.org/10.1186/s13075-014-0471-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.1101328", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041385958"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm.2426", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042197765", 
          "https://doi.org/10.1038/nm.2426"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/22.12.1077", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064086725"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/22.12.1077", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064086725"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017", 
    "datePublishedReg": "2017-01-01", 
    "description": "Follicular regulatory T cells are a subset of Foxp3+ regulatory T cells that migrate into the B cell follicle after infection or immunization and modulate the germinal center response. The anatomical positioning of follicular regulatory T cells within the germinal center is a defining characteristic of this subset of regulatory T cells; because of this, it is critical that studies of follicular regulatory T cells are able to identify them in situ. In this chapter we describe an immunofluorescence staining method to visualize follicular regulatory T cells in frozen secondary lymphoid tissue sections by confocal imaging.", 
    "editor": [
      {
        "familyName": "Calado", 
        "givenName": "Dinis Pedro", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-4939-7095-7_7", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2777680", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": {
      "isbn": [
        "978-1-4939-7094-0", 
        "978-1-4939-7095-7"
      ], 
      "name": "Germinal Centers", 
      "type": "Book"
    }, 
    "name": "Identifying Follicular Regulatory T Cells by Confocal Microscopy", 
    "pagination": "87-93", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-4939-7095-7_7"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "05456eda7409ca487b28b0e4a6405836e1322c0cdb35a60174e33c005f01ae0e"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1085906577"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28589349"
        ]
      }
    ], 
    "publisher": {
      "location": "New York, NY", 
      "name": "Springer New York", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-4939-7095-7_7", 
      "https://app.dimensions.ai/details/publication/pub.1085906577"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-15T12:13", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000600.jsonl", 
    "type": "Chapter", 
    "url": "http://link.springer.com/10.1007/978-1-4939-7095-7_7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7095-7_7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7095-7_7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7095-7_7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7095-7_7'


 

This table displays all metadata directly associated to this object as RDF triples.

136 TRIPLES      23 PREDICATES      43 URIs      30 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-4939-7095-7_7 schema:about N00ec8f8a3c864ceb8ab8182df325380f
2 N0cdd122e58f349aa9f4fc5b898e272e9
3 N16c42ac5b961480ebaa4892804dcc2d6
4 N1f59e35660ec46eca1cbfc46230004dc
5 N759cd51edda34b71a2a432d8560f460e
6 N794f838179ea4f62b8571810d5d86187
7 N9f50827f82014ccb86acea74005d19c0
8 Nf0e03a33a3db4819bbd44262f14cab42
9 Nf0e88fe0e7ca460ca34e056a95abf104
10 anzsrc-for:06
11 anzsrc-for:0601
12 schema:author Ne979f00c76ca4f8ea1630f9f6a319869
13 schema:citation sg:pub.10.1038/nm.2425
14 sg:pub.10.1038/nm.2426
15 sg:pub.10.1186/s13075-014-0471-7
16 https://doi.org/10.1002/eji.201546094
17 https://doi.org/10.1177/22.12.1077
18 https://doi.org/10.4049/jimmunol.1101328
19 schema:datePublished 2017
20 schema:datePublishedReg 2017-01-01
21 schema:description Follicular regulatory T cells are a subset of Foxp3<sup>+</sup> regulatory T cells that migrate into the B cell follicle after infection or immunization and modulate the germinal center response. The anatomical positioning of follicular regulatory T cells within the germinal center is a defining characteristic of this subset of regulatory T cells; because of this, it is critical that studies of follicular regulatory T cells are able to identify them in situ. In this chapter we describe an immunofluorescence staining method to visualize follicular regulatory T cells in frozen secondary lymphoid tissue sections by confocal imaging.
22 schema:editor N992657ecdc2f4ef9851462986c12043f
23 schema:genre chapter
24 schema:inLanguage en
25 schema:isAccessibleForFree false
26 schema:isPartOf N7cdb7f6231914f6682f1d5c05e77533b
27 schema:name Identifying Follicular Regulatory T Cells by Confocal Microscopy
28 schema:pagination 87-93
29 schema:productId N504e1cff5d984d01aefeed8c31910519
30 N51fdcd266b824c98b45dcaaf4b3d2f06
31 N5974bb26614d4445b985963555843224
32 N877da25a58d6404d8d0927077a39006a
33 schema:publisher Nd064953615f44d55bbe1aa57f27df73e
34 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085906577
35 https://doi.org/10.1007/978-1-4939-7095-7_7
36 schema:sdDatePublished 2019-04-15T12:13
37 schema:sdLicense https://scigraph.springernature.com/explorer/license/
38 schema:sdPublisher N4a3dc579ccf449c7bc736b8cc29566c1
39 schema:url http://link.springer.com/10.1007/978-1-4939-7095-7_7
40 sgo:license sg:explorer/license/
41 sgo:sdDataset chapters
42 rdf:type schema:Chapter
43 N00ec8f8a3c864ceb8ab8182df325380f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
44 schema:name Spleen
45 rdf:type schema:DefinedTerm
46 N0cdd122e58f349aa9f4fc5b898e272e9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
47 schema:name Microscopy, Confocal
48 rdf:type schema:DefinedTerm
49 N16c42ac5b961480ebaa4892804dcc2d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
50 schema:name Mice
51 rdf:type schema:DefinedTerm
52 N1f59e35660ec46eca1cbfc46230004dc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
53 schema:name Animals
54 rdf:type schema:DefinedTerm
55 N38ca24fcb13e4ea6aac7d97af68663b4 schema:name Lymphocyte Signalling and Development, Babraham Institute, Babraham Research Campus
56 rdf:type schema:Organization
57 N4a3dc579ccf449c7bc736b8cc29566c1 schema:name Springer Nature - SN SciGraph project
58 rdf:type schema:Organization
59 N504e1cff5d984d01aefeed8c31910519 schema:name pubmed_id
60 schema:value 28589349
61 rdf:type schema:PropertyValue
62 N51fdcd266b824c98b45dcaaf4b3d2f06 schema:name dimensions_id
63 schema:value pub.1085906577
64 rdf:type schema:PropertyValue
65 N5974bb26614d4445b985963555843224 schema:name readcube_id
66 schema:value 05456eda7409ca487b28b0e4a6405836e1322c0cdb35a60174e33c005f01ae0e
67 rdf:type schema:PropertyValue
68 N695172b323ee4dd58295addcf0f84168 schema:familyName Calado
69 schema:givenName Dinis Pedro
70 rdf:type schema:Person
71 N759cd51edda34b71a2a432d8560f460e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Germinal Center
73 rdf:type schema:DefinedTerm
74 N794f838179ea4f62b8571810d5d86187 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name T-Lymphocyte Subsets
76 rdf:type schema:DefinedTerm
77 N7cdb7f6231914f6682f1d5c05e77533b schema:isbn 978-1-4939-7094-0
78 978-1-4939-7095-7
79 schema:name Germinal Centers
80 rdf:type schema:Book
81 N7e3a40029ca9428787377b822aaa249c schema:name Lymphocyte Signalling and Development, Babraham Institute, Babraham Research Campus
82 rdf:type schema:Organization
83 N877da25a58d6404d8d0927077a39006a schema:name doi
84 schema:value 10.1007/978-1-4939-7095-7_7
85 rdf:type schema:PropertyValue
86 N992657ecdc2f4ef9851462986c12043f rdf:first N695172b323ee4dd58295addcf0f84168
87 rdf:rest rdf:nil
88 N9f50827f82014ccb86acea74005d19c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Biomarkers
90 rdf:type schema:DefinedTerm
91 Nb208590f444c46b9b1acc38e7efd92c5 rdf:first sg:person.01137254350.67
92 rdf:rest rdf:nil
93 Nd064953615f44d55bbe1aa57f27df73e schema:location New York, NY
94 schema:name Springer New York
95 rdf:type schema:Organisation
96 Ne979f00c76ca4f8ea1630f9f6a319869 rdf:first sg:person.0631204154.61
97 rdf:rest Nb208590f444c46b9b1acc38e7efd92c5
98 Nf0e03a33a3db4819bbd44262f14cab42 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name T-Lymphocytes, Regulatory
100 rdf:type schema:DefinedTerm
101 Nf0e88fe0e7ca460ca34e056a95abf104 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Fluorescent Antibody Technique
103 rdf:type schema:DefinedTerm
104 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
105 schema:name Biological Sciences
106 rdf:type schema:DefinedTerm
107 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
108 schema:name Biochemistry and Cell Biology
109 rdf:type schema:DefinedTerm
110 sg:grant.2777680 http://pending.schema.org/fundedItem sg:pub.10.1007/978-1-4939-7095-7_7
111 rdf:type schema:MonetaryGrant
112 sg:person.01137254350.67 schema:affiliation N38ca24fcb13e4ea6aac7d97af68663b4
113 schema:familyName Linterman
114 schema:givenName Michelle A.
115 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137254350.67
116 rdf:type schema:Person
117 sg:person.0631204154.61 schema:affiliation N7e3a40029ca9428787377b822aaa249c
118 schema:familyName Vanderleyden
119 schema:givenName Ine
120 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0631204154.61
121 rdf:type schema:Person
122 sg:pub.10.1038/nm.2425 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016362145
123 https://doi.org/10.1038/nm.2425
124 rdf:type schema:CreativeWork
125 sg:pub.10.1038/nm.2426 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042197765
126 https://doi.org/10.1038/nm.2426
127 rdf:type schema:CreativeWork
128 sg:pub.10.1186/s13075-014-0471-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033648211
129 https://doi.org/10.1186/s13075-014-0471-7
130 rdf:type schema:CreativeWork
131 https://doi.org/10.1002/eji.201546094 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019099760
132 rdf:type schema:CreativeWork
133 https://doi.org/10.1177/22.12.1077 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064086725
134 rdf:type schema:CreativeWork
135 https://doi.org/10.4049/jimmunol.1101328 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041385958
136 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...