Ontology type: schema:Chapter
2017
AUTHORSGregor L. Weiss , João M. Medeiros , Martin Pilhofer
ABSTRACTThe unique property of electron cryotomography (ECT) is its capability to resolve the structure of macromolecular machines in their cellular context. The integration of ECT data with high-resolution structures of purified subcomplexes and live-cell fluorescence light microscopy can generate pseudo-atomic models that lead to a mechanistic understanding across size and time scales. Recent advances in electron detection, sample thinning, data acquisition, and data processing have significantly enhanced the applicability and performance of ECT. Here we describe a detailed workflow for an ECT experiment, including cell culture, vitrification, data acquisition, data reconstruction, tomogram analysis, and subtomogram averaging. This protocol provides an entry point to the technique for students and researchers and indicates the many possible variations arising from specific target properties and the available instrumentation. More... »
PAGES353-375
Bacterial Protein Secretion Systems
ISBN
978-1-4939-7031-5
978-1-4939-7033-9
http://scigraph.springernature.com/pub.10.1007/978-1-4939-7033-9_27
DOIhttp://dx.doi.org/10.1007/978-1-4939-7033-9_27
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/28667625
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curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7033-9_27'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7033-9_27'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-7033-9_27'
This table displays all metadata directly associated to this object as RDF triples.
308 TRIPLES
23 PREDICATES
95 URIs
26 LITERALS
14 BLANK NODES