Flow Cytometric Analysis of the Expression Pattern of Peroxisomal Proteins, Abcd1, Abcd2, and Abcd3 in BV-2 Murine Microglial Cells View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2017

AUTHORS

Meryam Debbabi , Thomas Nury , Imen Helali , El Mostafa Karym , Flore Geillon , Catherine Gondcaille , Doriane Trompier , Amina Najid , Sébastien Terreau , Maryem Bezine , Amira Zarrouk , Anne Vejux , Pierre Andreoletti , Mustapha Cherkaoui-Malki , Stéphane Savary , Gérard Lizard

ABSTRACT

Microglial cells play important roles in neurodegenerative diseases including peroxisomal leukodystrophies. The BV-2 murine immortalized cells are widely used in the context of neurodegenerative researches. It is therefore important to establish the expression pattern of peroxisomal proteins by flow cytometry in these cells. So, the expression pattern of various peroxisomal transporters (Abcd1, Abcd2, Abcd3) contributing to peroxisomal β-oxidation was evaluated on BV-2 cells by flow cytometry and complementary methods (fluorescence microscopy, and RT-qPCR). By flow cytometry a strong expression of peroxisomal proteins (Abcd1, Abcd2, Abcd3) was observed. These data were in agreement with those obtained by fluorescence microscopy (presence of numerous fluorescent dots in the cytoplasm characteristic of a peroxisomal staining pattern) and RT-qPCR (high levels of Abcd1, Abcd2, and Abcd3 mRNAs). Thus, the peroxisomal proteins (Abcd1, Abcd2, Abcd3) are expressed in BV-2 cells, and can be analyzed by flow cytometry. More... »

PAGES

257-265

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-6937-1_25

DOI

http://dx.doi.org/10.1007/978-1-4939-6937-1_25

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084851587

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28409470


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "ATP-Binding Cassette Transporters", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flow Cytometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microglia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Fluorescence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peroxisomes", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Monastir", 
          "id": "https://www.grid.ac/institutes/grid.411838.7", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9", 
            "Facult\u00e9 de M\u00e9decine, Laboratoire de Nutrition\u2014Aliments Fonctionnels et Sant\u00e9 Vasculaire (LR12ES05), Monastir & Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Monastir"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Debbabi", 
        "givenName": "Meryam", 
        "id": "sg:person.01203554212.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01203554212.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Burgundy", 
          "id": "https://www.grid.ac/institutes/grid.5613.1", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nury", 
        "givenName": "Thomas", 
        "id": "sg:person.01250162517.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250162517.91"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Monastir", 
          "id": "https://www.grid.ac/institutes/grid.411838.7", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9", 
            "Facult\u00e9 de Pharmacie, Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives (LR99ES27), Universit\u00e9 de Monastir"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Helali", 
        "givenName": "Imen", 
        "id": "sg:person.01272527157.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272527157.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universit\u00e9 Hassan 1er", 
          "id": "https://www.grid.ac/institutes/grid.440487.b", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9", 
            "Laboratoire de Biochimie et Neuroscience, Facult\u00e9 de Sciences et Techniques, Universit\u00e9 Hassan 1er"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Karym", 
        "givenName": "El Mostafa", 
        "id": "sg:person.01130574105.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130574105.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Burgundy", 
          "id": "https://www.grid.ac/institutes/grid.5613.1", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Geillon", 
        "givenName": "Flore", 
        "id": "sg:person.01242410764.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242410764.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Burgundy", 
          "id": "https://www.grid.ac/institutes/grid.5613.1", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gondcaille", 
        "givenName": "Catherine", 
        "id": "sg:person.01335212675.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335212675.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Burgundy", 
          "id": "https://www.grid.ac/institutes/grid.5613.1", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Trompier", 
        "givenName": "Doriane", 
        "id": "sg:person.01033150513.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033150513.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Burgundy", 
          "id": "https://www.grid.ac/institutes/grid.5613.1", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Najid", 
        "givenName": "Amina", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Burgundy", 
          "id": "https://www.grid.ac/institutes/grid.5613.1", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Terreau", 
        "givenName": "S\u00e9bastien", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Burgundy", 
          "id": "https://www.grid.ac/institutes/grid.5613.1", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9", 
            "Laboratoire de Venins et Biomol\u00e9cules Th\u00e9rapeutiques (LVMT), Universit\u00e9 de Tunis El Manar-Institut Pasteur"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bezine", 
        "givenName": "Maryem", 
        "id": "sg:person.016471510621.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016471510621.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Monastir", 
          "id": "https://www.grid.ac/institutes/grid.411838.7", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9", 
            "Facult\u00e9 de M\u00e9decine, Laboratoire de Nutrition\u2014Aliments Fonctionnels et Sant\u00e9 Vasculaire (LR12ES05), Monastir & Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Monastir"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zarrouk", 
        "givenName": "Amira", 
        "id": "sg:person.01225005763.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01225005763.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Burgundy", 
          "id": "https://www.grid.ac/institutes/grid.5613.1", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vejux", 
        "givenName": "Anne", 
        "id": "sg:person.0653270615.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653270615.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Burgundy", 
          "id": "https://www.grid.ac/institutes/grid.5613.1", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Andreoletti", 
        "givenName": "Pierre", 
        "id": "sg:person.01072040706.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01072040706.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Burgundy", 
          "id": "https://www.grid.ac/institutes/grid.5613.1", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cherkaoui-Malki", 
        "givenName": "Mustapha", 
        "id": "sg:person.01003341717.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003341717.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Burgundy", 
          "id": "https://www.grid.ac/institutes/grid.5613.1", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Savary", 
        "givenName": "St\u00e9phane", 
        "id": "sg:person.01132023464.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132023464.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Burgundy", 
          "id": "https://www.grid.ac/institutes/grid.5613.1", 
          "name": [
            "Laboratoire \u2018Biochimie du peroxysome, inflammation et m\u00e9tabolisme lipidique\u2019, EA7270/INSERM, Facult\u00e9 des Sciences Gabriel, Universit\u00e9 de Bourgogne Franche Comt\u00e9"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lizard", 
        "givenName": "G\u00e9rard", 
        "id": "sg:person.01014163740.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01014163740.42"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.bbamem.2010.07.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000965726"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.74.082803.133329", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002979897"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbadis.2011.12.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006384866"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1742-2094-9-61", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016348794", 
          "https://doi.org/10.1186/1742-2094-9-61"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biochi.2013.08.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018737907"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng2070", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018769518", 
          "https://doi.org/10.1038/ng2070"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00418-012-0941-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021568484", 
          "https://doi.org/10.1007/s00418-012-0941-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1750-3639.2010.00390.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026097176"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1750-3639.2010.00390.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026097176"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1476-5381.2011.01435.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033816549"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1194/jlr.m900237-jlr200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039109459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jnr.490310405", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041500937"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jnr.490310405", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041500937"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/hmg/ddt645", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042027771"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/nbdi.1997.0127", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045558837"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbadis.2012.03.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046641824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jemt.10326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053295622"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.2009.06311.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053431658"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.2009.06311.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053431658"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.14573/altex.2009.2.83", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067341468"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4103/2152-7806.118349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072246297"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4103/2152-7806.118349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072246297"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017", 
    "datePublishedReg": "2017-01-01", 
    "description": "Microglial cells play important roles in neurodegenerative diseases including peroxisomal leukodystrophies. The BV-2 murine immortalized cells are widely used in the context of neurodegenerative researches. It is therefore important to establish the expression pattern of peroxisomal proteins by flow cytometry in these cells. So, the expression pattern of various peroxisomal transporters (Abcd1, Abcd2, Abcd3) contributing to peroxisomal \u03b2-oxidation was evaluated on BV-2 cells by flow cytometry and complementary methods (fluorescence microscopy, and RT-qPCR). By flow cytometry a strong expression of peroxisomal proteins (Abcd1, Abcd2, Abcd3) was observed. These data were in agreement with those obtained by fluorescence microscopy (presence of numerous fluorescent dots in the cytoplasm characteristic of a peroxisomal staining pattern) and RT-qPCR (high levels of Abcd1, Abcd2, and Abcd3 mRNAs). Thus, the peroxisomal proteins (Abcd1, Abcd2, Abcd3) are expressed in BV-2 cells, and can be analyzed by flow cytometry.", 
    "editor": [
      {
        "familyName": "Schrader", 
        "givenName": "Michael", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-4939-6937-1_25", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-4939-6935-7", 
        "978-1-4939-6937-1"
      ], 
      "name": "Peroxisomes", 
      "type": "Book"
    }, 
    "name": "Flow Cytometric Analysis of the Expression Pattern of Peroxisomal Proteins, Abcd1, Abcd2, and Abcd3 in BV-2 Murine Microglial Cells", 
    "pagination": "257-265", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-4939-6937-1_25"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "fa2e172421a88d32393c7fbab6c12f3b5ed13e5fa218f4c36c3a9058c244476b"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1084851587"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28409470"
        ]
      }
    ], 
    "publisher": {
      "location": "New York, NY", 
      "name": "Springer New York", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-4939-6937-1_25", 
      "https://app.dimensions.ai/details/publication/pub.1084851587"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-15T12:46", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000393.jsonl", 
    "type": "Chapter", 
    "url": "http://link.springer.com/10.1007/978-1-4939-6937-1_25"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-6937-1_25'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-6937-1_25'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-6937-1_25'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-4939-6937-1_25'


 

This table displays all metadata directly associated to this object as RDF triples.

279 TRIPLES      23 PREDICATES      56 URIs      31 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-4939-6937-1_25 schema:about N013dd1bc613143dd8ea5ac9f446b22d2
2 N0611173f65a043da8bb9c603de314b9c
3 N0f111d4b29fd474a980c3a1524764473
4 N21a477fb1d07406f9f3b2f977e50b6ac
5 N6298096b9c504f5f8808beb7a88eb3fd
6 N7446abf09e394ba998f7c25cd2c00cbb
7 N7b822f9e71a0466db5f065e3f8bba744
8 Nc9ea3be874794a3a8947d98bdb6ae6f3
9 Nd0df1b4d09b04c46862a047ea2ebebfc
10 Nf832ba21461e40118c8b11c57f6b32cd
11 anzsrc-for:06
12 anzsrc-for:0601
13 schema:author Ncdb4bc01f22548caa8c3ed5ea2b6a429
14 schema:citation sg:pub.10.1007/s00418-012-0941-4
15 sg:pub.10.1038/ng2070
16 sg:pub.10.1186/1742-2094-9-61
17 https://doi.org/10.1002/jemt.10326
18 https://doi.org/10.1002/jnr.490310405
19 https://doi.org/10.1006/nbdi.1997.0127
20 https://doi.org/10.1016/j.bbadis.2011.12.001
21 https://doi.org/10.1016/j.bbadis.2012.03.003
22 https://doi.org/10.1016/j.bbamem.2010.07.020
23 https://doi.org/10.1016/j.biochi.2013.08.022
24 https://doi.org/10.1093/hmg/ddt645
25 https://doi.org/10.1111/j.1471-4159.2009.06311.x
26 https://doi.org/10.1111/j.1476-5381.2011.01435.x
27 https://doi.org/10.1111/j.1750-3639.2010.00390.x
28 https://doi.org/10.1146/annurev.biochem.74.082803.133329
29 https://doi.org/10.1194/jlr.m900237-jlr200
30 https://doi.org/10.14573/altex.2009.2.83
31 https://doi.org/10.4103/2152-7806.118349
32 schema:datePublished 2017
33 schema:datePublishedReg 2017-01-01
34 schema:description Microglial cells play important roles in neurodegenerative diseases including peroxisomal leukodystrophies. The BV-2 murine immortalized cells are widely used in the context of neurodegenerative researches. It is therefore important to establish the expression pattern of peroxisomal proteins by flow cytometry in these cells. So, the expression pattern of various peroxisomal transporters (Abcd1, Abcd2, Abcd3) contributing to peroxisomal β-oxidation was evaluated on BV-2 cells by flow cytometry and complementary methods (fluorescence microscopy, and RT-qPCR). By flow cytometry a strong expression of peroxisomal proteins (Abcd1, Abcd2, Abcd3) was observed. These data were in agreement with those obtained by fluorescence microscopy (presence of numerous fluorescent dots in the cytoplasm characteristic of a peroxisomal staining pattern) and RT-qPCR (high levels of Abcd1, Abcd2, and Abcd3 mRNAs). Thus, the peroxisomal proteins (Abcd1, Abcd2, Abcd3) are expressed in BV-2 cells, and can be analyzed by flow cytometry.
35 schema:editor N4bbeb885f8bd4613addc9d62f12dfe07
36 schema:genre chapter
37 schema:inLanguage en
38 schema:isAccessibleForFree false
39 schema:isPartOf N8c577130e3b44e60bde95edea749e743
40 schema:name Flow Cytometric Analysis of the Expression Pattern of Peroxisomal Proteins, Abcd1, Abcd2, and Abcd3 in BV-2 Murine Microglial Cells
41 schema:pagination 257-265
42 schema:productId N126016ca4f3946adaf5f848b337948ea
43 N83bc348549fc432a95627981ef27f2d3
44 Nc935adffc0af4741aaadc73ba066d5be
45 Nda4e7feff7e340fe97f1a2a89486519c
46 schema:publisher N09ae6b8d1a364c80976342be031eb314
47 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084851587
48 https://doi.org/10.1007/978-1-4939-6937-1_25
49 schema:sdDatePublished 2019-04-15T12:46
50 schema:sdLicense https://scigraph.springernature.com/explorer/license/
51 schema:sdPublisher N806334c85826421da2076251791e1b78
52 schema:url http://link.springer.com/10.1007/978-1-4939-6937-1_25
53 sgo:license sg:explorer/license/
54 sgo:sdDataset chapters
55 rdf:type schema:Chapter
56 N00e861550cca4da4b23e7187734bc41f schema:affiliation https://www.grid.ac/institutes/grid.5613.1
57 schema:familyName Najid
58 schema:givenName Amina
59 rdf:type schema:Person
60 N013dd1bc613143dd8ea5ac9f446b22d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
61 schema:name ATP-Binding Cassette Transporters
62 rdf:type schema:DefinedTerm
63 N0611173f65a043da8bb9c603de314b9c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
64 schema:name Cloning, Molecular
65 rdf:type schema:DefinedTerm
66 N09ae6b8d1a364c80976342be031eb314 schema:location New York, NY
67 schema:name Springer New York
68 rdf:type schema:Organisation
69 N0f111d4b29fd474a980c3a1524764473 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name Animals
71 rdf:type schema:DefinedTerm
72 N126016ca4f3946adaf5f848b337948ea schema:name pubmed_id
73 schema:value 28409470
74 rdf:type schema:PropertyValue
75 N1437cb1228b94083b6e4ceb45ca0c0e8 rdf:first sg:person.01003341717.31
76 rdf:rest Nb203e3b81b8d4a4dba5d952d557fa03a
77 N17553b2938084a829bd2f074bab52b9a rdf:first sg:person.0653270615.54
78 rdf:rest N3a281fea68de49a8ba1710104a0a8deb
79 N18c73448d2b34887a5dc7b856735bd50 rdf:first sg:person.01242410764.67
80 rdf:rest Nef270182aa35429581e93b23c6b91116
81 N1b8b1d996d8843f6b1ff052e7563a25e rdf:first sg:person.01250162517.91
82 rdf:rest N9eef3618bcf94146a10801d64581b866
83 N21a477fb1d07406f9f3b2f977e50b6ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Mice
85 rdf:type schema:DefinedTerm
86 N26c00fa52ded4686be88805da2c81f2a rdf:first sg:person.01014163740.42
87 rdf:rest rdf:nil
88 N345ec64e6c6b455a9bb57afeb2c92181 rdf:first Na7adc54629254245803f77644f105615
89 rdf:rest N4caa1246e8074d54947d21852dedb565
90 N3a281fea68de49a8ba1710104a0a8deb rdf:first sg:person.01072040706.24
91 rdf:rest N1437cb1228b94083b6e4ceb45ca0c0e8
92 N3ae3091248054d31aba275bf629d9dfb schema:familyName Schrader
93 schema:givenName Michael
94 rdf:type schema:Person
95 N3d8827ca5fe74631ae5219463fa551ea rdf:first sg:person.01033150513.09
96 rdf:rest N4f5ec8c7d41248708c609f1dc7cd5a47
97 N4bbeb885f8bd4613addc9d62f12dfe07 rdf:first N3ae3091248054d31aba275bf629d9dfb
98 rdf:rest rdf:nil
99 N4caa1246e8074d54947d21852dedb565 rdf:first sg:person.016471510621.57
100 rdf:rest N8101adf0b234471ba44f385e09554de9
101 N4f5ec8c7d41248708c609f1dc7cd5a47 rdf:first N00e861550cca4da4b23e7187734bc41f
102 rdf:rest N345ec64e6c6b455a9bb57afeb2c92181
103 N6298096b9c504f5f8808beb7a88eb3fd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Peroxisomes
105 rdf:type schema:DefinedTerm
106 N709b63a5c32c44b78ab4587d3e85a0ff rdf:first sg:person.01130574105.03
107 rdf:rest N18c73448d2b34887a5dc7b856735bd50
108 N7446abf09e394ba998f7c25cd2c00cbb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Cell Line
110 rdf:type schema:DefinedTerm
111 N7b822f9e71a0466db5f065e3f8bba744 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Microscopy, Fluorescence
113 rdf:type schema:DefinedTerm
114 N806334c85826421da2076251791e1b78 schema:name Springer Nature - SN SciGraph project
115 rdf:type schema:Organization
116 N8101adf0b234471ba44f385e09554de9 rdf:first sg:person.01225005763.77
117 rdf:rest N17553b2938084a829bd2f074bab52b9a
118 N83bc348549fc432a95627981ef27f2d3 schema:name doi
119 schema:value 10.1007/978-1-4939-6937-1_25
120 rdf:type schema:PropertyValue
121 N8c577130e3b44e60bde95edea749e743 schema:isbn 978-1-4939-6935-7
122 978-1-4939-6937-1
123 schema:name Peroxisomes
124 rdf:type schema:Book
125 N9eef3618bcf94146a10801d64581b866 rdf:first sg:person.01272527157.25
126 rdf:rest N709b63a5c32c44b78ab4587d3e85a0ff
127 Na7adc54629254245803f77644f105615 schema:affiliation https://www.grid.ac/institutes/grid.5613.1
128 schema:familyName Terreau
129 schema:givenName Sébastien
130 rdf:type schema:Person
131 Nb203e3b81b8d4a4dba5d952d557fa03a rdf:first sg:person.01132023464.54
132 rdf:rest N26c00fa52ded4686be88805da2c81f2a
133 Nc935adffc0af4741aaadc73ba066d5be schema:name dimensions_id
134 schema:value pub.1084851587
135 rdf:type schema:PropertyValue
136 Nc9ea3be874794a3a8947d98bdb6ae6f3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Flow Cytometry
138 rdf:type schema:DefinedTerm
139 Ncdb4bc01f22548caa8c3ed5ea2b6a429 rdf:first sg:person.01203554212.58
140 rdf:rest N1b8b1d996d8843f6b1ff052e7563a25e
141 Nd0df1b4d09b04c46862a047ea2ebebfc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Gene Expression
143 rdf:type schema:DefinedTerm
144 Nda4e7feff7e340fe97f1a2a89486519c schema:name readcube_id
145 schema:value fa2e172421a88d32393c7fbab6c12f3b5ed13e5fa218f4c36c3a9058c244476b
146 rdf:type schema:PropertyValue
147 Nef270182aa35429581e93b23c6b91116 rdf:first sg:person.01335212675.22
148 rdf:rest N3d8827ca5fe74631ae5219463fa551ea
149 Nf832ba21461e40118c8b11c57f6b32cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Microglia
151 rdf:type schema:DefinedTerm
152 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
153 schema:name Biological Sciences
154 rdf:type schema:DefinedTerm
155 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
156 schema:name Biochemistry and Cell Biology
157 rdf:type schema:DefinedTerm
158 sg:person.01003341717.31 schema:affiliation https://www.grid.ac/institutes/grid.5613.1
159 schema:familyName Cherkaoui-Malki
160 schema:givenName Mustapha
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003341717.31
162 rdf:type schema:Person
163 sg:person.01014163740.42 schema:affiliation https://www.grid.ac/institutes/grid.5613.1
164 schema:familyName Lizard
165 schema:givenName Gérard
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01014163740.42
167 rdf:type schema:Person
168 sg:person.01033150513.09 schema:affiliation https://www.grid.ac/institutes/grid.5613.1
169 schema:familyName Trompier
170 schema:givenName Doriane
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033150513.09
172 rdf:type schema:Person
173 sg:person.01072040706.24 schema:affiliation https://www.grid.ac/institutes/grid.5613.1
174 schema:familyName Andreoletti
175 schema:givenName Pierre
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01072040706.24
177 rdf:type schema:Person
178 sg:person.01130574105.03 schema:affiliation https://www.grid.ac/institutes/grid.440487.b
179 schema:familyName Karym
180 schema:givenName El Mostafa
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130574105.03
182 rdf:type schema:Person
183 sg:person.01132023464.54 schema:affiliation https://www.grid.ac/institutes/grid.5613.1
184 schema:familyName Savary
185 schema:givenName Stéphane
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132023464.54
187 rdf:type schema:Person
188 sg:person.01203554212.58 schema:affiliation https://www.grid.ac/institutes/grid.411838.7
189 schema:familyName Debbabi
190 schema:givenName Meryam
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01203554212.58
192 rdf:type schema:Person
193 sg:person.01225005763.77 schema:affiliation https://www.grid.ac/institutes/grid.411838.7
194 schema:familyName Zarrouk
195 schema:givenName Amira
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01225005763.77
197 rdf:type schema:Person
198 sg:person.01242410764.67 schema:affiliation https://www.grid.ac/institutes/grid.5613.1
199 schema:familyName Geillon
200 schema:givenName Flore
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242410764.67
202 rdf:type schema:Person
203 sg:person.01250162517.91 schema:affiliation https://www.grid.ac/institutes/grid.5613.1
204 schema:familyName Nury
205 schema:givenName Thomas
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250162517.91
207 rdf:type schema:Person
208 sg:person.01272527157.25 schema:affiliation https://www.grid.ac/institutes/grid.411838.7
209 schema:familyName Helali
210 schema:givenName Imen
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272527157.25
212 rdf:type schema:Person
213 sg:person.01335212675.22 schema:affiliation https://www.grid.ac/institutes/grid.5613.1
214 schema:familyName Gondcaille
215 schema:givenName Catherine
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335212675.22
217 rdf:type schema:Person
218 sg:person.016471510621.57 schema:affiliation https://www.grid.ac/institutes/grid.5613.1
219 schema:familyName Bezine
220 schema:givenName Maryem
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016471510621.57
222 rdf:type schema:Person
223 sg:person.0653270615.54 schema:affiliation https://www.grid.ac/institutes/grid.5613.1
224 schema:familyName Vejux
225 schema:givenName Anne
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653270615.54
227 rdf:type schema:Person
228 sg:pub.10.1007/s00418-012-0941-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021568484
229 https://doi.org/10.1007/s00418-012-0941-4
230 rdf:type schema:CreativeWork
231 sg:pub.10.1038/ng2070 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018769518
232 https://doi.org/10.1038/ng2070
233 rdf:type schema:CreativeWork
234 sg:pub.10.1186/1742-2094-9-61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016348794
235 https://doi.org/10.1186/1742-2094-9-61
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1002/jemt.10326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053295622
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1002/jnr.490310405 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041500937
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1006/nbdi.1997.0127 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045558837
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/j.bbadis.2011.12.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006384866
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/j.bbadis.2012.03.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046641824
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1016/j.bbamem.2010.07.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000965726
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1016/j.biochi.2013.08.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018737907
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1093/hmg/ddt645 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042027771
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1111/j.1471-4159.2009.06311.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1053431658
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1111/j.1476-5381.2011.01435.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1033816549
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1111/j.1750-3639.2010.00390.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1026097176
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1146/annurev.biochem.74.082803.133329 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002979897
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1194/jlr.m900237-jlr200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039109459
262 rdf:type schema:CreativeWork
263 https://doi.org/10.14573/altex.2009.2.83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1067341468
264 rdf:type schema:CreativeWork
265 https://doi.org/10.4103/2152-7806.118349 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072246297
266 rdf:type schema:CreativeWork
267 https://www.grid.ac/institutes/grid.411838.7 schema:alternateName University of Monastir
268 schema:name Faculté de Médecine, Laboratoire de Nutrition—Aliments Fonctionnels et Santé Vasculaire (LR12ES05), Monastir & Faculté de Médecine, Université de Monastir
269 Faculté de Pharmacie, Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives (LR99ES27), Université de Monastir
270 Laboratoire ‘Biochimie du peroxysome, inflammation et métabolisme lipidique’, EA7270/INSERM, Faculté des Sciences Gabriel, Université de Bourgogne Franche Comté
271 rdf:type schema:Organization
272 https://www.grid.ac/institutes/grid.440487.b schema:alternateName Université Hassan 1er
273 schema:name Laboratoire de Biochimie et Neuroscience, Faculté de Sciences et Techniques, Université Hassan 1er
274 Laboratoire ‘Biochimie du peroxysome, inflammation et métabolisme lipidique’, EA7270/INSERM, Faculté des Sciences Gabriel, Université de Bourgogne Franche Comté
275 rdf:type schema:Organization
276 https://www.grid.ac/institutes/grid.5613.1 schema:alternateName University of Burgundy
277 schema:name Laboratoire de Venins et Biomolécules Thérapeutiques (LVMT), Université de Tunis El Manar-Institut Pasteur
278 Laboratoire ‘Biochimie du peroxysome, inflammation et métabolisme lipidique’, EA7270/INSERM, Faculté des Sciences Gabriel, Université de Bourgogne Franche Comté
279 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...