A Simplified and Systematic Method to Isolate, Culture, and Characterize Multiple Types of Human Dental Stem Cells from a Single ... View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2017

AUTHORS

Mohammed Bakkar , Younan Liu , Dongdong Fang , Camille Stegen , Xinyun Su , Murali Ramamoorthi , Li-Chieh Lin , Takako Kawasaki , Nicholas Makhoul , Huan Pham , Yoshinori Sumita , Simon D. Tran

ABSTRACT

This chapter describes a simplified method that allows the systematic isolation of multiple types of dental stem cells such as dental pulp stem cells (DPSC), periodontal ligament stem cells (PDLSC), and stem cells of the apical papilla (SCAP) from a single tooth. Of specific interest is the modified laboratory approach to harvest/retrieve the dental pulp tissue by minimizing trauma to DPSC by continuous irrigation, reduction of frictional heat from the bur rotation, and reduction of the bur contact time with the dentin. Also, the use of a chisel and a mallet will maximize the number of live DPSC for culture. Steps demonstrating the potential for multiple cell differentiation lineages of each type of dental stem cell into either osteocytes, adipocytes, or chondrocytes are described. Flow cytometry, with a detailed strategy for cell gating and analysis, is described to verify characteristic markers of human mesenchymal multipotent stromal cells (MSC) from DPSC, PDLSC, or SCAP for subsequent experiments in cell therapy and in tissue engineering. Overall, this method can be adapted to any laboratory with a general setup for cell culture experiments. More... »

PAGES

191-207

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-6756-8_15

DOI

http://dx.doi.org/10.1007/978-1-4939-6756-8_15

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1083859538

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28229417


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