Isolation of Chromoplasts and Suborganellar Compartments from Tomato and Bell Pepper Fruit View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2017

AUTHORS

Cristina Barsan , Marcel Kuntz , Jean-Claude Pech

ABSTRACT

Tomato is a model for fruit development and ripening. The isolation of intact plastids from this organism is therefore important for metabolic and proteomic analyses. Pepper, a species from the same family, is also of interest since it allows isolation of intact chromoplasts in large amounts. Here, we provide a detailed protocol for the isolation of tomato plastids at three fruit developmental stages, namely, nascent chromoplasts from the mature green stage, chromoplasts from an intermediate stage, and fully differentiated red chromoplasts. The method relies on sucrose density gradient centrifugations. It yields high purity organelles suitable for proteome analyses. Enzymatic and microscopy assays are summarized to assess purity and intactness. A method is also described for subfractionation of pepper chromoplast lipoprotein structures. More... »

PAGES

61-71

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-6533-5_5

DOI

http://dx.doi.org/10.1007/978-1-4939-6533-5_5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049684037

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27730602


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