In Vitro Analysis of Tem1 GTPase Activity and Regulation by the Bfa1/Bub2 GAP View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2017

AUTHORS

Marco Geymonat , Adonis Spanos , Katrin Rittinger

ABSTRACT

Tem1 is a small GTPase that controls the mitotic progression of Saccharomyces cerevisiae through the Mitotic Exit Network. Tem1 activity is tightly controlled in mitosis by Bub2 and Bfa1 and is also regulated by the spindle orientation checkpoint that monitors the correct alignment of the mitotic spindle with the mother-daughter axis. In this chapter we describe the purification of Tem1, Bfa1, and Bub2 and a detailed radioactive filter-binding assay to study the nucleotide binding properties of Tem1 and the role of its regulators Bfa1 and Bub2. More... »

PAGES

71-80

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-6502-1_6

DOI

http://dx.doi.org/10.1007/978-1-4939-6502-1_6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018349270

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27826857


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