CRISPR/Cas9 Genome Editing in Embryonic Stem Cells View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2017

AUTHORS

Guillaume Andrey , Malte Spielmann

ABSTRACT

Targeted mutagenesis is required to evaluate the function of DNA segments across the genome. In recent years the CRISPR/Cas9 technology has been widely used for functional genome studies and is partially replacing classical homologous recombination methods in different aspects. CRISPR/Cas9-derived tools indeed allow the production of a wide-range of engineered mutations: from point mutations to large chromosomal rearrangements such as deletions, duplications and inversions. Here we present a protocol to engineer Embryonic Stem Cells (ESC) with desired mutations using transfection of custom-made CRISPR/Cas9 vectors. These methods allow the in vivo modeling of congenital mutations and the functional interrogation of DNA sequences. More... »

PAGES

221-34

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-4035-6_15

DOI

http://dx.doi.org/10.1007/978-1-4939-4035-6_15

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043854580

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27662879


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