Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2016

AUTHORS

Ming Chi , Basdeo Bhagwat , Guiliang Tang , Yu Xiang

ABSTRACT

It is of great importance and interest to develop crop varieties with low polyphenol oxidase (PPO) activity for the food industry because PPO-mediated oxidative browning is a main cause of post-harvest deterioration and quality loss of fresh produce and processed foods. We recently demonstrated that potato tubers with reduced browning phenotypes can be produced by inhibition of the expression of several PPO gene isoforms using artificial microRNA (amiRNA) technology. The approach introduces a single type of 21-nucleotide RNA population to guide silencing of the PPO gene transcripts in potato tissues. Some advantages of the technology are: small RNA molecules are genetically transformed, off-target gene silencing can be avoided or minimized at the stage of amiRNA designs, and accuracy and efficiency of the processes can be detected at every step using molecular biological techniques. Here we describe the methods for transformation and regeneration of potatoes with amiRNA vectors, detection of the expression of amiRNAs, identification of the cleaved product of the target gene transcripts, and assay of the expression level of PPO gene isoforms in potatoes. More... »

PAGES

163-78

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-3393-8_15

DOI

http://dx.doi.org/10.1007/978-1-4939-3393-8_15

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035345817

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26843174


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