Oral Rabies Vaccine Design for Expression in Plants View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2016-04-14

AUTHORS

Ankit Singh , Gauri Saxena , Praveen C. Verma

ABSTRACT

Vaccination is the sensitization process of the immune system against any pathogen. Generally, recombinant subunit vaccines are considered safer than attenuated vaccines. As whole pathogenic organisms are used in the immunization process, the attenuated vaccines are considered more risky than subunit vaccines. Rabies is the oldest known zoonosis which spreads through a neurotropic Lyssavirus primarily mediated through infected canine bites. Rabies causes worldwide loss of more than 60,000 human lives every year. Animal vaccination is equally important to check the transmission of rabies into humans. Rabies oral vaccination can be a good alternative where multiple booster and priming regimens are required while the painful vaccination process can continue for long durations. Introduction of oral vaccines was made to ease the discomfort associated with the mode of introduction of conventional vaccines into the body. Although the rabies oral vaccine can substantially reduce the cost of vaccination in the developing countries, mass immunization programs need larger quantities of vaccines which should be delivered at nominal cost. Expression of recombinant antigen proteins in E. coli is often not viable because of lack of post-translational modifications and folding requirements. Though yeast and insect cell line expression systems have post-translational processing and modifications, significantly different immunological response against their post-translational modification pattern limits their deployment as an expression system. As an alternative, plants are emerging as a promising system to express and deliver wide range of functionally active biopharmaceutical product at lower cost for mass immunization programs. As generation of vaccine antigenic proteins in plant systems are cheaper, the strategy will benefit developing countries where this disease causes thousands of deaths every year. In this chapter, we will discuss about our efforts toward development of oral rabies vaccine and the methodological steps involved during this procedure in detail. More... »

PAGES

547-567

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-3389-1_36

DOI

http://dx.doi.org/10.1007/978-1-4939-3389-1_36

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022082662

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27076322


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