Molecular Analyses of Transgenic Plants View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2016

AUTHORS

Kurniawan Rudi Trijatmiko , Felichi Mae Arines , Norman Oliva , Inez Hortense Slamet-Loedin , Ajay Kohli

ABSTRACT

One of the major challenges in plant molecular biology is to generate transgenic plants that express transgenes stably over generations. Here, we describe some routine methods to study transgene locus structure and to analyze transgene expression in plants: Southern hybridization using DIG chemiluminescent technology for characterization of transgenic locus, SYBR Green-based real-time RT-PCR to measure transgene transcript level, and protein immunoblot analysis to evaluate accumulation and stability of transgenic protein product in the target tissue. More... »

PAGES

201-222

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-3289-4_15

DOI

http://dx.doi.org/10.1007/978-1-4939-3289-4_15

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003875371

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26614292


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