Luciferase Reporter Assays to Study Transcriptional Activity of Hedgehog Signaling in Normal and Cancer Cells View Full Text


Ontology type: schema:Chapter      Open Access: True


Chapter Info

DATE

2015

AUTHORS

Silvia Pandolfi , Barbara Stecca

ABSTRACT

The measurement of the transcriptional activity of the HH signaling pathway is widely used as an indication of pathway activation. Luciferase reporter assays are powerful tools to measure the specific ability of a transcription factor to bind to its consensus sequence and to activate transcription of target genes. Here, we describe a protocol to measure the transcriptional activity of the HH pathway in normal and cancer cells. This technique allows studying the activity of GLI transcription factors and their modulation by drugs and/or other factors. More... »

PAGES

71-9

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-2772-2_7

DOI

http://dx.doi.org/10.1007/978-1-4939-2772-2_7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001386961

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26179040


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166 rdf:type schema:CreativeWork
 




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