Multiplex Detection of Plant Pathogens Through the Luminex Magplex Bead System View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2015

AUTHORS

René A. A. van der Vlugt , Henry van Raaij , Marjanne de Weerdt , Jan H. W. Bergervoet

ABSTRACT

Here we describe a versatile multiplex method for both the serological and molecular detection of plant pathogens. The Luminex MagPlex bead system uses small paramagnetic microspheres ("beads"), either coated with specific antibodies or oligonucleotides, which capture respectively viruses and/or bacteria or PCR products obtained from their genetic material. The Luminex MagPlex bead system allows true multiplex detection of up to 500 targets in a single sample on a routine basis. The liquid suspension nature of the method significantly improves (1) assay speed, (2) detection limits and (3) dynamic range. It can also considerably reduce labor and consumables costs. More... »

PAGES

283-99

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-4939-2620-6_21

DOI

http://dx.doi.org/10.1007/978-1-4939-2620-6_21

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015630839

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25981262


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